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Author Ki Yong Choi ♦ Duck Kyun Choi ♦ Ga Young Han ♦ Dae Sung Yoon ♦ Tae Song Kim ♦ Sang Myung Lee ♦ Yoon-Sik Lee
Source IEEE Xplore Digital Library
Content type Text
Publisher Institute of Electrical and Electronics Engineers, Inc. (IEEE)
File Format PDF
Copyright Year ©2006
Language English
Subject Domain (in DDC) Technology ♦ Engineering & allied operations ♦ Applied physics
Subject Keyword Magnetosphere ♦ Magnetic resonance ♦ Biosensors ♦ Immune system ♦ Resonant frequency ♦ Chemical and biological sensors ♦ Instruments ♦ Magnetic sensors ♦ Sensor systems ♦ Frequency conversion
Abstract The sensitivity of immunoassays strongly depends on the binding affinity of the antibodies, which is also limited by a variety of other factors that are specific to the assay configuration, the type of label and instrument system used for detection. Magnetic beads are used in order to increase sensitivity in diagnosis system using the piezoelectric microcantilever. Use of magnetic beads is seemingly viable to insure the high sensitivity as well as the stable immunoassay. The ultra-sensitive magneto-mechanic detection of protein is accomplished by monitoring resonant frequency change of a cantilever functionalized with magnetic beads. The resonant frequency is surely shifted by mass and magnetic field effect. As a result, we practiced sandwich assay using CRP secondary antibody-coated magnetic bead on cantilever. Resonant frequency of the cantilever is changed by mass effect. When CRP secondary antibody-coated magnetic beads are attached to a CRP antigen, the changes in normalized resonant frequency shift $deltaf/f_{1}$ were found to definitely increase about 30 times.
Description Author affiliation: Hanyang Univ., Seoul (Ki Yong Choi; Duck Kyun Choi)
ISBN 1424403758
ISSN 19300395
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research ♦ Reading
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2006-10-22
Publisher Place South Korea
Rights Holder Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Size (in Bytes) 3.85 MB
Page Count 4
Starting Page 148
Ending Page 151


Source: IEEE Xplore Digital Library