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Author Heitz, J. ♦ Reisinger, B. ♦ Yakunin, S. ♦ Voelcker, N. ♦ Qi Peng ♦ Hook, A.L. ♦ Romanin, C. ♦ Fahrner, M.
Source IEEE Xplore Digital Library
Content type Text
Publisher Institute of Electrical and Electronics Engineers, Inc. (IEEE)
File Format PDF
Copyright Year ©2011
Language English
Subject Domain (in DDC) Technology ♦ Engineering & allied operations ♦ Applied physics
Subject Keyword Electrodes ♦ Polymers ♦ Positron emission tomography ♦ Surface emitting lasers ♦ Nanobioscience ♦ Surface topography ♦ electroporation ♦ laser-induced nano-structures ♦ polymer surfaces ♦ ripples ♦ biological cells
Abstract We describe the formation of laser-induced photonic micro- and nanostructures at polyethylene terephthalate (PET) surfaces, which can be used for applications in cell biology. Two types of laser-induced periodic structures are investigated in detail: (1) structures with dimensions of a few µm originating from relaxation of stress fields in laser-irradiated polymer surfaces and (2) sub-wavelength ripple-type structures formed by interference of the incident laser-beam with scattered light at the surface. We demonstrate that both types of structures are able to induce an alignment of living biological cells cultured thereon. In order to study mammalian cell cultures there is a need to perform biological assays, e.g., for introduction of genetically active material into cells. This process is known as cell transfection, which can be performed by electroporation exposing the cells to an electrical field gradient. In the current paper, we demonstrate that laser-induced micro-or nano-structures on a polymer surface between 100 µm wide gold lead to biological cells oriented either parallel or perpendicular to the electrodes. Finally, we discuss the potential of these laser-patterned polymer electroporation chips for enhanced sorting selectivity and better transfection efficiency.
Description Author affiliation: Institute of Applied Physics, Johannes Kepler University Linz, Altenbergerstr. 69, 4040, Austria (Heitz, J.; Reisinger, B.; Yakunin, S.)
ISBN 9781457708817
ISSN 21612064
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research ♦ Reading
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2011-06-26
Publisher Place Sweden
Rights Holder Institute of Electrical and Electronics Engineers, Inc. (IEEE)
e-ISBN 9781457708824
Size (in Bytes) 1.34 MB
Page Count 4
Starting Page 1
Ending Page 4

Source: IEEE Xplore Digital Library