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Author Khin Moh Moh Aung ♦ Naim, A. ♦ Xiaodi Su
Source IEEE Xplore Digital Library
Content type Text
Publisher Institute of Electrical and Electronics Engineers, Inc. (IEEE)
File Format PDF
Copyright Year ©2009
Language English
Subject Domain (in DDC) Technology ♦ Engineering & allied operations ♦ Applied physics
Subject Keyword Biochemistry ♦ Plasmons ♦ Resonance ♦ Erbium ♦ Sequences ♦ DNA ♦ Proteins ♦ Nuclear measurements ♦ Protocols ♦ Recruitment
Abstract We have applied SPR imaging technique for efficient and precise measurement of the binding of estrogen receptors (ERα and ERβ) with their response elements (EREs) arrayed on chip. Four EREs - one containing a perfect core sequence (GGTCAnnnTGACC), and three containing imperfect cores (i.e., with one base substitution in one of the half-site, GGTCAnnnTGGCC; with one half-site mutant, GGTCAnnnCACGA and a scrambled DNA with both half-site mutant) were involved in this study. ERα and ERβ binding to these sequences at two buffer conditions (higher and lower ionic strength) was measured to determine the salt effects and sequence impact on ER-ERE binding. Distinct binding behaviour of ERα and ERβ has been compared. Conventional SPR experiments were run in parallel to cross check the results from SPR imager and to optimize the assay protocol of the SPR imager. A precise measurement of sequence dependent ER-ERE binding is of significance in determining the rules, by which ERs are recruited by DNA.
Description Author affiliation: Institute of Materials Research and Engineering, 3 Research Link, Singapore 117602 (Khin Moh Moh Aung; Naim, A.; Xiaodi Su)
ISBN 9781424445486
ISSN 19300395
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research ♦ Reading
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2009-10-25
Publisher Place New Zealand
Rights Holder Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Size (in Bytes) 1.45 MB
Page Count 6
Starting Page 347
Ending Page 352

Source: IEEE Xplore Digital Library