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Author Karczemska, A. ♦ Sokolowska, A.
Sponsorship KEiT
Source IEEE Xplore Digital Library
Content type Text
Publisher Institute of Electrical and Electronics Engineers, Inc. (IEEE)
File Format PDF
Copyright Year ©2001
Language English
Subject Domain (in DDC) Technology ♦ Engineering & allied operations ♦ Applied physics
Subject Keyword Electromagnetic wave absorption ♦ DNA ♦ Electrokinetics ♦ Fluorescence ♦ Modems ♦ Optical materials ♦ Manufacturing ♦ Microfluidics ♦ Biological materials ♦ Optical polymers
Abstract Summary form only given. The rapid progress in the field of microfluidic devices, among them capillary electrophoresis microchips for DNA sequencing, stimulates a desire to exploit further the possibilities of such devices. Modem DNA sequencers are automated, small and allow very rapid separations (up to seconds). Different detection techniques are applied into the existing systems, among them the most common is fluorescence. However, a novel technique, based on the intrinsic absorption in nucleic acids of ultraviolet (UV) light, will solve a lot of detection problems. With regard to the advantages of the absorption technique, a material to manufacture a DNA sequencer should transmit UV light in the range of 260 nm where nucleic acids absorb strongly. Selection of material is therefore limited to wide band gap materials. Presented here are investigations focused on the optimization of a DNA chip. Different materials such as diamond, sapphire, polymers and quartz are compared with regard to optical and electrical properties, biological and chemical inertness and heat transfer Different phenomena occurring during capillary electrophoretic separations are taken into consideration. The advantages and drawbacks of using different materials are discussed, taking into consideration the technological problems of manufacturing microstructures and economical aspects.
Description Author affiliation: Inst. of Turbomachinery, Tech. Univ. of Lodz, Poland (Karczemska, A.)
ISBN 0780371364
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research ♦ Reading
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2001-06-26
Publisher Place Poland
Rights Holder Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Size (in Bytes) 80.29 kB


Source: IEEE Xplore Digital Library