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Author Dae-Sik Lee ♦ Jeong Won Park ♦ Nae-Lim Lee ♦ Mun Yeon Jung ♦ Sung-Mok Cho
Source IEEE Xplore Digital Library
Content type Text
Publisher Institute of Electrical and Electronics Engineers, Inc. (IEEE)
File Format PDF
Copyright Year ©2013
Language English
Subject Domain (in DDC) Technology ♦ Engineering & allied operations ♦ Applied physics
Subject Keyword Magnetic separation ♦ Blood ♦ Cancer ♦ Wires ♦ Microfluidics ♦ Tumors
Abstract This paper presents the design and fabrication of the microdevice for separation of circulating tumor cells (CTCs) from human whole blood based on lateral magnetophoresis principle and immunomagnetic nanobeads with the antiepithelial cell adhesive molecule (EpCAM) antibodies that selectively bind to epithelial cancer cells. We designed damascene v-shaped 60 μm-thick Nickel-Cobalt (Ni-Co) ferromagnetic wires with a flat zone and fabricated with micromachining technologies. The separation is carried out through lateral magnetophoresis, stimulated by magnetic field gradient-based isolation technologies. Experimentally, first, we observed the effect of external static magnetic intensity on the isolation rates. And we showed that microdevices isolates about 93% of the spiked CTCs cancer cells (MCF-7, a breast cancer cell line) in human whole blood at a flow rate of 40/100 μL/min with respect to human whole blood/buffer solution. For the overall isolation, it takes 15 min to process and analyze 500 μL of human whole blood.
Description Author affiliation: Next Generation Display Res. Dept., ETRI, Daejeon, South Korea (Sung-Mok Cho) || BT Convergence Res. Dept., Electron. Telecommun. Res. Inst. (ETRI), Daejeon, South Korea (Dae-Sik Lee; Jeong Won Park; Nae-Lim Lee; Mun Yeon Jung)
ISBN 9781467346429
ISSN 19300395
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Research ♦ Reading
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-11-03
Publisher Place USA
Rights Holder Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Size (in Bytes) 1.22 MB
Page Count 4
Starting Page 1
Ending Page 4


Source: IEEE Xplore Digital Library