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Author Arozal, Wawaimuli ♦ Dhewayani, Radiana ♦ Dwiyono, Supraja
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher Universitas Indonesia
File Format HTM / HTML
Date Created 2017-10-04
Copyright Year ©2016
Language English ♦ Indonesian
Subject Domain (in LCC) R ♦ R5-920
Subject Keyword Medicine
Abstract Penggunaan doksorubisin (DOX) sebagai antikanker dapat menyebabkan efek samping di organ lainseperti testis akibat peningkatan stres oksidatif. Penelitian ini bertujuan untuk mengetahui efek antioksidanmangiferin yang terkandung dalam Mangifera indica untuk mengurangi toksisitas testis. Sebanyak 24 ekortikus jantan sprague dawley, dibagi menjadi empat kelompok. Kelompok normal mendapatkan injeksi NaCl0,9%, kelompok kontrol negatif mendapatkan DOX total 15mg/kgBB; kelompok DOX mendapat mangiferin30mg/kgBB dan mangiferin 60mg/kgBB. Setelah 7 minggu, tikus dimatikan dan testis dikumpulkan untukanalisis parameter stres oksidatif biokimia yaitu kadar malonedyaldehide (MDA), aktivitas superoxidedysmutase (SOD), perubahan histologi dan apoptosis kaspase-9 dan kaspase-12. Pemberian mangiferindosis 30mg/kgBB dan 60mg/kgBB selama 7 minggu dapat mengurangi kerusakan sel spermatogenik dansel sertoli tubulus seminiferus testis, menurunkan kadar MDA dan menurunkan ekspresi kaspase-9 padakelompok yang diberikan DOX dan mangiferin. Perbaikan parameter tersebut mengindikasikan mangiferinmempunyai efek proteksi terhadap kerusakan sel spematogenik dan sel sertoli tubulus seminiferus testis tikusyang diberikan DOX. Kata kunci: toksisitas doksorubisin, stres oksidatif, apoptosis, mangiferin, testis. Mangiferin Effects against Doxorubicin Toxicity in Rat Testis Abstract The use of doxorubicin (DOX) as an anti-cancer agent might cause side effects to other organs suchas testicle due to increased oxidative stress. This study was aimed to investigate the antioxidant effect ofmangiferin inside Mangifera indica for lowering testicle toxicity. A total of 24 sprague dawley male rats wasdivided into 4 groups. The normal group was injected with 0.9% of NaCl, the negative control group receivedDOX with dosage of 15 mg/BW while DOX treated group received mangiferin with dosage of 30 mg/BW and60 mg/BW. After 7 weeks, all rats were killed and testicles from every rats were subsequently collected foroxidative stress parameter analysis which were measuring the rate of malonedyaldehide (MDA), superoxidedysmutase activity, histological changes, and apoptosis of caspase-9 and caspase-12. Administration ofmangiferin with dosage of 30 mg/BW and 60 mg/BW for 7 weeks decreased the destruction of spermatogeniccells and sertoli cells of seminiferous tubule, decreased the rate of MDA, and lowered the expression ofcaspase-9 in the group which received DOX and mangiferin. Improvement in those parameters indicated thatmangiferin had a protective effect towards spermatogenic and sertoli cells of seminiferous tubule destructionin rats which were given DOX. Keywords: doxorubicin toxicity, oxidative stress, apoptosis, mangiferin, testicles.
ISSN 23381426
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2016-09-01
e-ISSN 23381426
Journal Journal Kedokteran Indonesia
Volume Number 4
Issue Number 2
Page Count 94
Starting Page 104
Ending Page 11


Source: Directory of Open Access Journals (DOAJ)