|Source||United States Department of Energy Office of Scientific and Technical Information|
|Publisher||Nature Publishing Group|
|Subject Keyword||BIOLOGY AND MEDICINE ♦ AMINO ACIDS ♦ AMPHIBIANS ♦ ANIMALS ♦ EMBRYOS ♦ FROGS ♦ HYBRIDS ♦ METABOLISM ♦ NUCLEIC ACIDS ♦ RADIOAUTOGRAPHY ♦ TRACER TECHNIQUES ♦ TRITIUM|
|Abstract||Two-cell stages of hybrid embryos resulting from the cross-fertilization of Bufo and Rana temporaria were incubated for 17 hrs in a medium containing tritium-labeled thymidine. The embryos were fixed by freeze-substitution and the incorporation of tritium studied by the radioautographic technique. The embryos stopped development at the late blastula stage. Labeling of desoxyribonucleic acid was demonstrated in morula as well as in blastula cells of the lethal hybrids. Tritium-labeled thymidine was shown to be incorporated into desoxyribonucleic acid 24 hr after development stopped, which suggests that the block in development was not due to the arrest of desoxyribonucleic acid synthesis. (C.H.)|
|Learning Resource Type||Article|
|Publisher Department||Universite Libre, Brussels|
|Organization||Universite Libre, Brussels|
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