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Author TENCER, B.
Sponsorship USDOE
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Publisher Nature Publishing Group
Language English
Subject Keyword BIOLOGY AND MEDICINE ♦ AMINO ACIDS ♦ AMPHIBIANS ♦ ANIMALS ♦ EMBRYOS ♦ FROGS ♦ HYBRIDS ♦ METABOLISM ♦ NUCLEIC ACIDS ♦ RADIOAUTOGRAPHY ♦ TRACER TECHNIQUES ♦ TRITIUM
Abstract Two-cell stages of hybrid embryos resulting from the cross-fertilization of Bufo and Rana temporaria were incubated for 17 hrs in a medium containing tritium-labeled thymidine. The embryos were fixed by freeze-substitution and the incorporation of tritium studied by the radioautographic technique. The embryos stopped development at the late blastula stage. Labeling of desoxyribonucleic acid was demonstrated in morula as well as in blastula cells of the lethal hybrids. Tritium-labeled thymidine was shown to be incorporated into desoxyribonucleic acid 24 hr after development stopped, which suggests that the block in development was not due to the arrest of desoxyribonucleic acid synthesis. (C.H.)
ISSN 00280836
Educational Use Research
Learning Resource Type Article
Publisher Date 1961-04-01
Publisher Department Universite Libre, Brussels
Journal Nature
Volume Number 190
Issue Number 4770
Organization Universite Libre, Brussels


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