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Author Kamide, Yosuke ♦ Ishizuka, Tamotsu ♦ Tobo, Masayuki ♦ Tsurumaki, Hiroaki ♦ Aoki, Haruka ♦ Mogi, Chihiro ♦ Nakakura, Takashi ♦ Yatomi, Masakiyo ♦ Ono, Akihiro ♦ Koga, Yasuhiko ♦ Sato, Koichi ♦ Hisada, Takeshi ♦ Dobashi, Kunio ♦ Yamada, Masanobu ♦ Okajima, Fumikazu
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ACIDIFICATION ♦ ALBUMINS ♦ ANTIGENS ♦ BLOOD SERUM ♦ BONE MARROW ♦ DINITROPHENOL ♦ ENVIRONMENT ♦ INFLAMMATION ♦ INHIBITION ♦ MAST CELLS ♦ MICE ♦ MUCOUS MEMBRANES ♦ PH VALUE ♦ PROTONS ♦ SKIN ♦ SURFACES
Abstract Although blood pH is maintained in a narrow range of around pH 7.4 in living organisms, inflammatory loci are characterized by acidic conditions. Mast cells tend to reside close to the surface of the body in areas such as the mucosa and skin where they may be exposed to exogenous acids, and they play an important role in immune responses. However, little is known about the effects of extracellular acidification on the functions of mast cell. Here, we found that extracellular acidification increased the dinitrophenyl-conjugated human serum albumin (DNP-HSA)-induced production of interleukin (IL)-6 and IL-13 in MC/9 cells or bone marrow-derived mouse mast cells sensitized with anti-DNP IgE. Extracellular acidification also inhibited migration of MC/9 cells toward DNP-HSA. In addition, acidic pH stimulated antigen-induced activation of p38 mitogen-activated protein kinase (MAPK) and protein kinase B (Akt). These findings suggest that extracellular acidification augmented antigen/IgE-induced and FcεRI-mediated production of IL-6 and IL-13 in mast cells, and that this was associated with the enhancement of p38 MAPK and Akt activation. - Highlights: • Antigen-induced IL-6 and IL-13 production was augmented by acidic pH in mast cells. • Acidic pH-induced actions were associated with activation of p38 MAPK and Akt. • Inhibition of p38 MAPK and Akt attenuated cytokine responses to acidic pH. • Acidic pH effects are not attributable to actions of known proton-sensing GPCRs.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-08-28
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 464
Issue Number 3


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