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Author Abdullah, A. L. ♦ Tengerdy, R. P. ♦ Murphy, V. G.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BASIC BIOLOGICAL SCIENCES ♦ SINGLE CELL PROTEIN ♦ BIOSYNTHESIS ♦ STRAW ♦ FERMENTATION ♦ BENCH-SCALE EXPERIMENTS ♦ BIOREACTORS ♦ CANDIDA ♦ FUNGI ♦ OPERATION ♦ OPTIMIZATION ♦ PRODUCTIVITY ♦ REACTION KINETICS ♦ SEMIBATCH CULTURE ♦ BIOCONVERSION ♦ KINETICS ♦ MICROORGANISMS ♦ PLANTS ♦ SYNTHESIS ♦ YEASTS ♦ Microbiology
Abstract Optimal conditions for solid substrate fermentation of wheat straw with Chaetomium cellulolyticum in laboratory-scale stationary layer fermenters were developed. The best pretreatment for wheat straw was ammonia freeze explosion, followed by steam treatment, alkali treatment, and simple autoclaving. The optimal fermentation conditions were 80% (w/w) moisture content; incubation temperature of 37 degrees C; 2% (w/w) unwashed mycelial inoculum; aeration at 0.12 L/h/g; substrate thickness of 1 to 2 cm; and duration of three days. Technical parameters for this optimized fermentation were: degree of substance utilization, 27.2%; protein yield/substrate, 0.09 g; biomass yield/bioconverted substrate, 0.40 g; degree of bioconversion of total available sugars in the substrate, 60.5%; specific efficiency of bioconversion, 70.8%; and overall efficiency of biomass production from substrate, 42.7%. Mixed culturing of Candida utilis further increased biomass production by 20%. The best mode of fermentation was a semicontinuous fed-batch fermentation where one-half of the fermented material was removed at three-day intervals and replaced by fresh substrate. In this mode, protein production was 20% higher than in batch mode, protein productivity was maintained over 12 days, and sporulation was prevented. 10 references.
Educational Use Research
Learning Resource Type Article
Publisher Date 1985-01-01
Publisher Place United States
Journal Biotechnol. Bioeng.
Volume Number 27
Issue Number 1
Organization Colorado State Univ., Fort Collins


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