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Author Lin, Li-mei ♦ Xiong, Su-hui ♦ Zhao, Ling-jia ♦ Tang, Jie ♦ Zhang, Zhi-min ♦ Li, Ya-mei ♦ Zheng, Tao ♦ Xia, Bo-hou
Editor Calapai, Gioacchino
Source Hindawi
Content type Text
Publisher Hindawi
File Format PDF
Copyright Year ©2018
Language English
Abstract In this study, optimization of enzyme-assisted extraction, purification, characterization, and its bioactivities of polysaccharides from Hedyotis corymbosa (HCP) was investigated. It was found that the optimum extraction conditions were 3% of enzyme concentration (X 1), 30 of liquid-to-solid ratio (X 2), 56°C of extraction temperature (X 3), 200W of ultrasonic power (X 4), 10 min of extraction time (X 5), and 5 of pH value (X 6). Under optimum conditions, the experimental yield (4.10 ± 0.16%) was closed to the predicted value (4.02%). The crude HCP was further purified using DEAE-52 and Sephadex G-150 gel column, and a major polysaccharide fraction from HCP, designed as HCP-1a with molecular weight of 33.9 kDa, was obtained. The HCP and HCP-1a were characterized by chemical analysis, FT-IR, and HPLC. For antioxidant activities in vitro, HCP possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. In subsequent immunostimulatory studies, significantly decreased NO, IL-1β, and TNF-α concentrations were observed in both of HCP and HCP-1a treated RAW264.7 cells. Therefore, this study may indicate some insights into the application of polysaccharides from Hedyotis corymbosa as potential natural antioxidants and immunostimulants.
ISSN 1741427X
Learning Resource Type Article
Publisher Date 2018-11-14
Rights License This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
e-ISSN 17414288
Journal Evidence-Based Complementary and Alternative Medicine
Volume Number 2018
Page Count 10