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Author Hwang, Do Won ♦ Park, Kyung Min ♦ Shim, Hye-kyung ♦ Jin, Yeona ♦ Oh, Hyun Jeong ♦ Oh, So Won ♦ Lee, Song ♦ Youn, Hyewon ♦ Joung, Yoon Ki ♦ Lee, Hong J. ♦ Kim, Seung U. ♦ Park, Ki Dong ♦ Lee, Dong Soo
Source Paperity
Content type Text
Publisher Springer Berlin Heidelberg
File Format PDF ♦ HTM / HTML
Copyright Year ©2014
Subject Keyword Orthopedics ♦ Cardiology ♦ Oncology ♦ Nuclear medicine ♦ Imaging / radiology
Abstract Background Three-dimensional (3D) hydrogel-based stem cell therapies contribute to enhanced therapeutic efficacy in treating diseases, and determining the optimal mechanical strength of the hydrogel in vivo is important for therapeutic success. We evaluated the proliferation of human neural stem cells incorporated within in situ-forming hydrogels and compared the effect of hydrogels with different elastic properties in cell/hydrogel-xenografted mice. Methods The gelatin-polyethylene glycol-tyramine (GPT) hydrogel was fabricated through enzyme-mediated cross-linking reaction using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). Results The F3-effluc encapsulated within a soft 1,800 pascal (Pa) hydrogel and stiff 5,800 Pa hydrogel proliferated vigorously in a 24-well plate until day 8. In vitro and in vivo kinetics of luciferase activity showed a slow time-to-peak after d-luciferin administration in the stiff hydrogel. When in vivo proliferation of F3-effluc was observed up to day 21 in both the hydrogel group and cell-only group, F3-effluc within the soft hydrogel proliferated more vigorously, compared to the cells within the stiff hydrogel. Ki-67-specific immunostaining revealed highly proliferative F3-effluc with compactly distributed cell population inside the 1,800 Pa or 5,800 Pa hydrogel. Conclusions We examined the in vivo effectiveness of different elastic types of hydrogels encapsulating viable neural stem cells by successfully monitoring the proliferation of implanted stem cells incorporated within a 3D hydrogel scaffold.
Learning Resource Type Article
Publisher Date 2014-12-01
e-ISSN 2191219X
Journal EJNMMI Research
Volume Number 4
Issue Number 1