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Author Weber, S. ♦ Prakash, L.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT. ♦ BASIC BIOLOGICAL SCIENCES ♦ DNA ♦ GENETIC RADIATION EFFECTS ♦ GENES ♦ DNA SEQUENCING ♦ SACCHAROMYCES CEREVISIAE ♦ MUTATIONS ♦ AMINO ACIDS ♦ EXPERIMENTAL DATA ♦ GENETIC MAPPING ♦ NUCLEOTIDES ♦ PROTEINS ♦ ULTRAVIOLET RADIATION ♦ BIOLOGICAL EFFECTS ♦ BIOLOGICAL RADIATION EFFECTS ♦ CARBOXYLIC ACIDS ♦ DATA ♦ ELECTROMAGNETIC RADIATION ♦ FUNGI ♦ GENETIC EFFECTS ♦ INFORMATION ♦ MAPPING ♦ MICROORGANISMS ♦ NUCLEIC ACIDS ♦ NUMERICAL DATA ♦ ORGANIC ACIDS ♦ ORGANIC COMPOUNDS ♦ PLANTS ♦ RADIATION EFFECTS ♦ RADIATIONS ♦ SACCHAROMYCES ♦ STRUCTURAL CHEMICAL ANALYSIS ♦ YEASTS ♦ Radiation Effects on Microorganisms- Basic Studies- (-1987) ♦ Genetics- Tracer Techniques
Abstract The RAD6 gene of Saccharomyces cerevisiae is required for postreplication repair of UV-damaged DNA, for induced mutagenesis, and for sporulation. The authors have mapped the transcripts and determined the nucleotide sequence of the cloned RAD6 gene. The RAD6 gene encodes two transcripts of 0.98 and 0.86 kilobases which differ only in their 3' termini. The transcribed region contains an open reading frame of 516 nucleotides. The rad6-1 and rad6-3 mutant alleles, which the authors have cloned and sequenced, introduce amber and ochre nonsense mutations, respectively into the open reading frame, proving that it encodes the RAD6 protein. The RAD6 protein predicted by the nucleotide sequence is 172 amino acids long, has a molecular weight of 19,704, and contains 23.3% acidic and 11.6% basic residues. Its most striking feature is the highly acidic carboxyl terminus: 20 of the 23 terminal amino acids are acidic, including 13 consecutive aspartates. RAD6 protein thus resembles high mobility group proteins HMG-1 and HMG-2, which each contain a carboxyl-proximal tract of acidic amino acids. 48 references, 6 figures.
Educational Use Research
Learning Resource Type Article
Publisher Date 1985-01-01
Publisher Place United States
Volume Number 82
Organization Univ. of Rochester School of Medicine and Dentistry, NY


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