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Author Schrek, R. ♦ Stefani, S.
Sponsorship USDOE
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BIOLOGY AND MEDICINE ♦ ANTIGENS ♦ LEUCOCYTES ♦ LYMPHOCYTES ♦ MAN ♦ NITROGEN MUSTARD ♦ ORGANIC NITROGEN COMPOUNDS ♦ QUANTITY RATIO ♦ RADIATION DOSES ♦ RADIATION EFFECTS ♦ SURVIVAL TIME ♦ SUSPENSIONS ♦ TEMPERATURE ♦ TUBES ♦ VARIATIONS ♦ X RADIATION
Abstract S>Suspensions of leukocytes were prepared from normal human blood. PHA- P (2 mu l/ml) was added and the suspensions, in small test tubes, were irradiated with 75-2400 r and incubated at 37 deg C. After irradiation with 1200 r, 29% of the lymphocytes and transformed lymphocytes in the PHA-treated suspensions survived 6 days of incubation. In contrast, after irradiation with 150 r only 5.4% of the lymphocytes survived in control suspensions without PHA. The addition of nitrogen mustard 5 mu g/ml allowed the survival of about one- half of the lymphocytes and lymphoblastoid cells. In contrast, the concentration of HN2 killed all the lymphocytes in control suspensions without PHA. (P.C.H.)
Educational Use Research
Learning Resource Type Article
Publisher Date 1963-11-01
Publisher Department Veterans Administration Hospital, Hines, Ill.
Journal Nature
Volume Number 200
Organization Veterans Administration Hospital, Hines, Ill.


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