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Author Miyai, Shunsuke ♦ Itoh, Johbu ♦ Kajiya, Hanako ♦ Takekoshi, Susumu ♦ Osamura, Robert Yoshiyuki
Source J-STAGE
Content type Text
Publisher JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
Language English
Subject Keyword Pit-1 ♦ transcription factors ♦ gene silencing ♦ RNA interference ♦ small interfering RNAs
Abstract RNA interference (RNAi) provides a powerful method for gene silencing in eukaryotic cells. We report on an RNAi technique-induced Pit-1 gene silencing in a GH-secreting cell line MtT/S using duplexes of 21-nt RNAs. By immunocytochemical analysis, the decrease in Pit-1 immunoreactivity was observed in a fraction of siRNA transfected MtT/S cells, while actin and tubulin showed no change in expression following transfection. Moreover, the double staining of Pit-1 and GH indicated that GH production was reduced only in MtT/S cells in which Pit-1 immunoreactivity was decreased by transfection. These results suggested that functional specific knock-down of Pit-1 was induced by transfection of siRNA. Together with the defects of somatotropic lineage and hypoplastic phenotypes in Snell dwarf mice and the transfection experiments using Pit-1 antisense oligonucleotides, our experiments support the theory that Pit-1 plays a crucial role in GH gene expression in individual cells.
ISSN 00445991
Learning Resource Type Article
Publisher Date 2005-01-01
e-ISSN 13475800
Journal ACTA HISTOCHEMICA ET CYTOCHEMICA(ahc)
Volume Number 38
Issue Number 2
Page Count 8
Starting Page 107
Ending Page 114


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