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Author Hirasaki, Masataka ♦ Nakamura, Fumika ♦ Yamagishi, Kazuo ♦ Numamoto, Minori ♦ Shimada, Yukiko ♦ Uehashi, Keigo ♦ Muta, Shigeru ♦ Sugiyama, Minetaka ♦ Kaneko, Yoshinobu ♦ Kuhara, Satoru ♦ Harashima, Satoshi
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Elsevier
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Chemical engineering ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Eukaryota ♦ Organisms ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Genetic Phenomena ♦ Biological Sciences
Subject Keyword Discipline Biomedical Engineering ♦ Discipline Microbiology ♦ Gene Expression Profiling ♦ Methods ♦ Phosphoric Monoester Hydrolases ♦ Metabolism ♦ Saccharomyces Cerevisiae Proteins ♦ Saccharomyces Cerevisiae ♦ Classification ♦ Enzymology ♦ Gene Expression Regulation, Enzymologic ♦ Physiology ♦ Gene Expression Regulation, Fungal ♦ Gene Knockout Techniques ♦ Mutation ♦ Genetics ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Expression profiles of protein phosphatase (PPase) disruptants were analyzed by use of Pearson's correlation coefficient to find profiles that correlated with those of 316 Reference Gene (RG) disruptants harboring deletions in genes with known functions. Twenty-six Deltappase disruptants exhibited either a positive or negative correlation with 94 RG disruptants when the p value for Pearson's correlation coefficient was >0.2. Some of the predictions that arose from this analysis were tested experimentally and several new Delta ppase phenotypes were found. Notably, Delta sit4 and Delta siw14 disruptants exhibited hygromycin B sensitivity, Delta sit4 and Delta ptc1 disruptants grew slowly on glycerol medium, the Delta ptc1 disruptant was found to be sensitive to calcofluor white and congo red, while the Delta ppg1 disruptant was found to be sensitive to congo red. Because on-going analysis of expression profiles of Saccharomyces cerevisiae disruptants is rapidly generating new data, we suggest that the approach used in the present study to explore PPase function is also applicable to other genes.
Description Country affiliation: Japan
Author Affiliation: Hirasaki M ( Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.)
ISSN 13891723
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-05-01
Publisher Place Japan
e-ISSN 13474421
Journal Journal of Bioscience and Bioengineering
Volume Number 109
Issue Number 5


Source: WHO-Global Index Medicus