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Author Lan, Dongming ♦ Tai, Yan ♦ Shen, Yanfei ♦ Wang, Fanghua ♦ Yang, Bo ♦ Wang, Yonghua
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Elsevier
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Technology ♦ Medicine & health ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Chemical engineering ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Macromolecular Substances ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Biological Sciences
Subject Keyword Discipline Biomedical Engineering ♦ Discipline Microbiology ♦ Enzymes, Immobilized ♦ Metabolism ♦ Peptide Hydrolases ♦ Protein Engineering ♦ Methods ♦ Recombinant Proteins ♦ Isolation & Purification ♦ Cellulose ♦ Green Fluorescent Proteins ♦ Genetics ♦ Protein Binding ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Cellulose binding domain (CBD) fusion protease was employed to digest the CBD fusion protein. After cleavage, the tag free target proteins can be separated from the CBD tag and CBD fusion protease which still adsorbed to the cellulose by centrifugation. The green fluorescent protein was efficiently purified using this method.
Description Author Affiliation: Lan D ( School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, PR China.)
ISSN 13891723
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2012-04-01
Publisher Place Japan
e-ISSN 13474421
Journal Journal of Bioscience and Bioengineering
Volume Number 113
Issue Number 4

Source: WHO-Global Index Medicus