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Author Karim, Mohammad Minnatul ♦ Hisamoto, Tatsunori ♦ Matsunaga, Tetsuro ♦ Asahi, Yoko ♦ Noiri, Yuichiro ♦ Ebisu, Shigeyuki ♦ Kato, Akio ♦ Azakami, Hiroyuki
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Elsevier
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Chemical engineering ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Bacteria ♦ Organisms ♦ Stomatognathic Diseases ♦ Diseases ♦ Organic Chemicals ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Diagnosis ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physical Phenomena ♦ Cell Physiological Phenomena ♦ Genetic Phenomena ♦ Microbiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Biomedical Engineering ♦ Discipline Microbiology ♦ Bacterial Proteins ♦ Metabolism ♦ Biofilms ♦ Growth & Development ♦ Carbon-sulfur Lyases ♦ Eikenella Corrodens ♦ Physiology ♦ Periodontal Diseases ♦ Microbiology ♦ Genetics ♦ Drug Effects ♦ Ultrastructure ♦ Homoserine ♦ Analogs & Derivatives ♦ Pharmacology ♦ Lactones ♦ Microbial Viability ♦ Microscopy, Confocal ♦ Mutation ♦ Quorum Sensing ♦ Time Factors ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Previously, we reported that biofilm formation of Eikenella corrodens is regulated by autoinducer-2 (AI-2), based on observations that biofilm-forming efficiency of ΔluxS mutant was greater than that of the wild type (Azakami et al., J. Biosci. Bioeng., 102, 110-117, 2006). To determine whether the AI-2 molecule affects biofilm formation directly, we added purified AI-2 to luxS mutant and wild-type E. corrodens and compared biofilm formations by using a static assay. Results indicated that biofilm formation in E. corrodens was enhanced by the addition of AI-2. We also compared the biofilms formed by flow cell system for the luxS mutant and the wild type by using scanning electron microscopy and confocal laser scanning microscopy. The number of viable bacteria in the luxS mutant biofilm was dramatically reduced and more sparsely distributed than that of the wild type, which suggested that AI-2 might enhance the mature biofilm. Conversely, further analysis by modified confocal reflection microscopy indicated that the wild-type biofilm was matured earlier than that of the luxS mutant, and became thinner and more sparsely distributed with time. These data suggest that LuxS may facilitate the maturation and detachment of biofilm in E. corrodens.
Description Country affiliation: Japan
Author Affiliation: Karim MM ( Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.)
ISSN 13891723
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-09-01
Publisher Place Japan
e-ISSN 13474421
Journal Journal of Bioscience and Bioengineering
Volume Number 116
Issue Number 3


Source: WHO-Global Index Medicus