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Author Uchida, Ryoichiro ♦ Bhawal, Ujjal K. ♦ Kiba, Hideo ♦ Arai, Kiyoshi ♦ Tanimoto, Yasuhiro ♦ Kuboyama, Noboru ♦ Asakura, Tetsuo ♦ Nishiyama, Norihiro
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Elsevier
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Chemical engineering
Subject Domain (in MeSH) Musculoskeletal System ♦ Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Inorganic Chemicals ♦ Macromolecular Substances ♦ Carbohydrates ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Equipment and Supplies ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Musculoskeletal and Neural Physiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Biomedical Engineering ♦ Discipline Microbiology ♦ Bone Regeneration ♦ Physiology ♦ Fibroins ♦ Chemistry ♦ Osteoblasts ♦ Cytology ♦ Tissue Scaffolds ♦ Animals ♦ Biological Markers ♦ Metabolism ♦ Bone Density ♦ Collagen Type I ♦ Genetics ♦ Core Binding Factor Alpha 1 Subunit ♦ Femur ♦ Injuries ♦ Physiopathology ♦ Gene Expression ♦ Hydrophobic And Hydrophilic Interactions ♦ Male ♦ Osteocalcin ♦ Plasma Gases ♦ Proteoglycans ♦ Rabbits ♦ Receptors, Transforming Growth Factor Beta ♦ Signal Transduction ♦ Transforming Growth Factor Beta ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract We have recently identified plasma-irradiated silk fibroin (P-AF) as a key regulator of bone matrix properties and composition. Bone matrix properties were tested in 48 femur critical size defects (3.25 mm in diameter) with the expression of osteoblast specific genes at 1 and 2 weeks after surgery. The scaffolds were characterized by various states of techniques; the scanning electronic microcopy revealed the large sized pores in the aqueous-based silk fibroin (A-F) scaffold and showed no alteration into the architecture by the addition of plasma irradiation. The contact angle measurements confirmed the introduction of plasma helped to change the hydrophobic nature into hydrophilic. The histological analyses confirmed the presence of silk fibroin in scaffolds and newly formed bone around the scaffolds. Immunohistochemical examination revealed the increased expression pattern in a set of osteoblast specific genes (TGF-ß, TGF-ß type III receptor, Runx2, type I collagen and osteocalcin). These data were the first to show that the properties of bone matrix are regulated, specifically through Runx2 pathway in P-AF group. Thus, an employment of P-AF increases several compositional properties of bone, including increased bone matrix, mineral concentration, cortical thickness, and trabecular bone volume.
Description Country affiliation: Japan
Author Affiliation: Uchida R ( Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan.); Bhawal UK ( Department of Biochemistry, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan.); Kiba H ( Department of Oral Pathology, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan.); Arai K ( Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan.); Tanimoto Y ( Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan.); Kuboyama N ( University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan.); Asakura T ( Department of Biotechnology, Tokyo University of Agriculture and Technology, Nakacho 2-24-16, Koganei, Tokyo 184-8588, Japan.); Nishiyama N ( Department of Dental Biomaterials, Nihon University School of Dentistry at Matsudo, 870-1 Sakaecho Nishi 2, Matsudo, Chiba 271-8587, Japan. Electronic address: nishiyama.norihiro@nihon-u.ac.jp.)
ISSN 13891723
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2014-09-01
Publisher Place Japan
e-ISSN 13474421
Journal Journal of Bioscience and Bioengineering
Volume Number 118
Issue Number 3


Source: WHO-Global Index Medicus