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Author Stanton, Gregory B. ♦ Kohler, Shawn J. ♦ Boklweski, Jennifer ♦ Cameron, Judy L. ♦ Greenough, William T.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Nervous System ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Heterocyclic Compounds ♦ Carbohydrates ♦ Amino Acids, Peptides, and Proteins ♦ Biological Factors ♦ Chemicals and Drugs ♦ Diagnosis ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physical Phenomena ♦ Cell Physiological Phenomena ♦ Physiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Neurology ♦ Antigens ♦ Metabolism ♦ Motor Cortex ♦ Cytology ♦ Neuroglia ♦ Physiology ♦ Neurons ♦ Classification ♦ Proteoglycans ♦ Animals ♦ Bromodeoxyuridine ♦ Cell Differentiation ♦ Female ♦ Macaca Fascicularis ♦ Anatomy & Histology ♦ Macaca Mulatta ♦ Microscopy, Confocal ♦ Nerve Tissue Proteins ♦ Organogenesis ♦ Time Factors ♦ Journal Article ♦ Research Support, N.i.h., Extramural ♦ Research Support, Non-u.s. Gov't
Abstract We used confocal microscopy and immunohistochemistry (IHC) to look for new cells in the motor cortex of adult macaque monkeys that might form the cellular bases of improved brain function from exercise. Twenty-four female Macaca fascicularis monkeys divided into groups by age (10-12 years, 15-17 years), postexercise survival periods, and controls, received 10 weekly injections of the thymidine analog, bromodeoxyuridine (BrdU) to mark new cells. Sixteen monkeys survived 15 weeks (5 weeks postexercise) and 8 monkeys survived 27 weeks (12 weeks postexercise) after initial BrdU injections. Additionally, five Macaca mulatta female monkeys (â ¼5.5-7 years) received single injections of BrdU and survived 2 days, 2 weeks, and 6 weeks after BrdU injections. Neural and glial antibodies were used to identify new cell phenotypes and to look for changes in proportions of these cells with respect to time and experimental conditions. No BrdU(+) /DCx(+) cells were found but about 7.5% of new cells were calretinin-positive (Cr(+) ). BrdU(+) /GABA(+) (gamma-aminobutyric acid) cells were also found but no new Cr(+) or GABA(+) cells colabeled with a mature neuron marker, NeuN or chondroitin sulfate antibody, NG2. The proportion of new cells that were NG2(+) was about 85% for short and long survival monkeys of which two, newly described perivascular phenotypes (Pldv and Elu) and a small percentage of pericytes (2.5%) comprised 44% and 51% of the new NG2(+) cells, respectively. Proportions of NG2(+) phenotypes were affected by post-BrdU survival periods, monkey age, and possibly a postexercise sedentary period but no direct effect of exercise was found.
Description Author Affiliation: Stanton GB ( Beckman Institute, University of Illinois, Urbana, IL, 61801.)
ISSN 00219967
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2015-04-15
Publisher Place United States
e-ISSN 10969861
Journal Journal of Comparative Neurology
Volume Number 523
Issue Number 6


Source: WHO-Global Index Medicus