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Author Morita, Tomotake ♦ Ogura, Yuki ♦ Takashima, Masako ♦ Hirose, Naoto ♦ Fukuoka, Tokuma ♦ Imura, Tomohiro ♦ Kondo, Yukishige ♦ Kitamoto, Dai
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Elsevier
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Chemical engineering
Subject Domain (in MeSH) Plant Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Carbohydrates ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Genetic Phenomena ♦ Biological Sciences
Subject Keyword Discipline Biomedical Engineering ♦ Discipline Microbiology ♦ Glycolipids ♦ Biosynthesis ♦ Surface-active Agents ♦ Metabolism ♦ Ustilaginales ♦ Genes, Rrna ♦ Chemistry ♦ Plant Leaves ♦ Microbiology ♦ Genetics ♦ Isolation & Purification ♦ Yeasts ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract An ustilaginomycetous anamorphic yeast species isolated from the leaves of Saccharum officinarum (sugarcane) in Okinawa, Japan, was identified as a novel Pseudozyma species based on morphological and physiological aspects and molecular taxonomic analysis using the D1/D2 domains of the large subunit (26S) rRNA gene and the internal transcribed spacer 1 (ITS1)-5.8S-ITS2 regions. The name Pseudozyma churashimaensis sp. nov. was proposed for the novel species, with JCM 16988(T) as the type strain. Interestingly, P. churashimaensis was found to produce glycolipid biosurfactants, a mixture of mannosylerythritol lipids (MELs), including a novel tri-acetylated derivative (MEL-A2), from glucose. The observed critical micelle concentration (CMC) and the surface tension at CMC of MEL-A2 were 1.7 × 10â »6 M and 29.2 mN/m, respectively. Moreover, on a water-penetration scan, MEL-A2 efficiently formed different lyotropic liquid crystalline phases, including the lamella phase at a wide range of concentrations, indicating its excellent surface-active and self-assembling properties. The novel strain of the genus Pseudozyma should thus facilitate the application of glycolipid biosurfactants in combination with other MEL producers.
Description Country affiliation: Japan
Author Affiliation: Morita T ( Research Institute for Innovations in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, Higashi 1-1, Tsukuba, Ibaraki 305-8565, Japan.)
ISSN 13891723
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2011-08-01
Publisher Place Japan
e-ISSN 13474421
Journal Journal of Bioscience and Bioengineering
Volume Number 112
Issue Number 2

Source: WHO-Global Index Medicus