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Author Shi, Huilin ♦ Cui, Hongjuan ♦ Alam, Goleeta ♦ Gunning, William T. ♦ Nestor, Andrea ♦ Giovannucci, David ♦ Zhang, Ming ♦ Ding, Han-Fei
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Social sciences ♦ Social problems & services; associations ♦ Social welfare problems & services ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases
Subject Domain (in MeSH) Nervous System ♦ Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Heterocyclic Compounds ♦ Macromolecular Substances ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Cell Physiological Phenomena ♦ Genetic Phenomena ♦ Biological Sciences ♦ Health Care Quality, Access, and Evaluation ♦ Health Care
Subject Keyword Discipline Neurology ♦ Ganglia, Sympathetic ♦ Cytology ♦ Growth & Development ♦ Gene Expression Regulation, Developmental ♦ Physiology ♦ Intermediate Filament Proteins ♦ Metabolism ♦ Nerve Tissue Proteins ♦ Neuroglia ♦ Neurons ♦ Stem Cells ♦ Age Factors ♦ Animals ♦ Animals, Newborn ♦ Bromodeoxyuridine ♦ Cell Proliferation ♦ Fatty Acid-binding Proteins ♦ Female ♦ Ki-67 Antigen ♦ Male ♦ Mice ♦ Mice, Inbred C57bl ♦ Nestin ♦ S100 Proteins ♦ Tyrosine 3-monooxygenase ♦ Journal Article ♦ Research Support, N.i.h., Extramural
Abstract Sympathetic ganglia are primarily composed of noradrenergic neurons and satellite glial cells. Although both cell types originate from neural crest cells, the identities of the progenitor populations at intermediate stages of the differentiation process remain to be established. Here we report on the identification in vivo of glial and neuronal progenitor cells in postnatal sympathetic ganglia, by using mouse superior cervical ganglia as a model system. There are significant levels of cellular proliferation in mouse superior cervical ganglia during the first 18 days after birth. A majority of the proliferating cells express both nestin and brain lipid-binding protein (BLBP). Bromodeoxyuridine (BrdU) fate-tracing experiments demonstrate that these nestin and BLBP double-positive cells represent a population of glial progenitors for sympathetic satellite cells. The glial differentiation process is characterized by a marked downregulation of nestin and upregulation of S100, with no significant changes in the levels of BLBP expression. We also identify a small number of proliferating cells that express nestin and tyrosine hydroxylase, a key enzyme of catecholamine biosynthesis that defines sympathetic noradrenergic neurons. Together, these results establish nestin as a common marker for sympathetic neuronal and glial progenitor cells and delineate the cellular basis for the generation and maturation of sympathetic satellite cells.
Description Country affiliation: United States
Author Affiliation: Shi H ( Department of Biochemistry and Cancer Biology, University of Toledo Health Science Campus, Toledo, Ohio 43614, USA.)
ISSN 00219967
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2008-06-20
Publisher Place United States
e-ISSN 10969861
Journal Journal of Comparative Neurology
Volume Number 508
Issue Number 6

Source: WHO-Global Index Medicus