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Author Zollinger, Therese ♦ Mertz, Kirsten D. ♦ Schmid, Mirka ♦ Schmitt, Anja ♦ Pfaltz, Madeleine ♦ Kempf, Werner
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Integumentary System ♦ Anatomy ♦ Eukaryota ♦ Bacteria ♦ Organisms ♦ Bacterial Infections and Mycoses ♦ Skin and Connective Tissue Diseases ♦ Diseases ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment
Subject Keyword Discipline Pathology ♦ Discipline Dermatology ♦ Borrelia Infections ♦ Pathology ♦ Borrelia ♦ Genetics ♦ Granuloma Annulare ♦ Microbiology ♦ Lichen Sclerosus Et Atrophicus ♦ Scleroderma, Localized ♦ Skin ♦ Complications ♦ Humans ♦ Polymerase Chain Reaction ♦ Journal Article
Abstract BACKGROUND: Morphea, granuloma annulare (GA) and lichen sclerosus et atrophicans (LSA) have also been suggested to be linked to Borrelia infection. Previous studies based on serologic data or detection of Borrelia by immunohistochemistry and polymerase chain reaction (PCR) reported contradictory results. Thus, we examined skin biopsies of morphea, GA and LSA by PCR to assess the prevalence of Borrelia DNA in an endemic area and to compare our results with data in the literature. METHODS: Amplification of DNA sequences of Borrelia burgdorferi sensu lato by nested PCR from formalin-fixed and paraffin-embedded skin biopsies of morphea, GA and LSA, followed by automated sequencing of amplification products. PCR-based studies on Borrelia species in these disorders published until July 2009 were retrieved by a literature search. RESULTS: Borrelia DNA was detected in 3 of 112 skin biopsies (2.7%) including one of 49 morphea biopsies (2.0%), one of 48 GA biopsies (2.1%) and one of 15 LSA biopsies (6.6%). Amplification products belonged to B. burgdorferi sensu stricto in two cases available for sequence analysis. CONCLUSIONS: The results of our and most of other PCR-based studies do not argue for a significant association of B. burgdorferi sensu lato with morphea, GA, LSA.
Description Country affiliation: Switzerland
Author Affiliation: Zollinger T ( Kempf und Pfaltz Histologische Diagnostik, Zürich, Switzerland.)
ISSN 03036987
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-05-01
Publisher Place United States
e-ISSN 16000560
Journal Journal of Cutaneous Pathology
Volume Number 37
Issue Number 5


Source: WHO-Global Index Medicus