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Author Mateju, Kristýna ♦ Sumová, Alena ♦ Bendová, Zdenka
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Sense Organs ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physical Phenomena ♦ Physiological Phenomena ♦ Reproductive and Urinary Physiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Neurology ♦ Light ♦ Period Circadian Proteins ♦ Proto-oncogene Proteins C-fos ♦ Retina ♦ Physiology ♦ Animals ♦ Animals, Newborn ♦ Biological Clocks ♦ Circadian Rhythm ♦ Darkness ♦ Female ♦ Male ♦ Mice ♦ Mice, Inbred C57bl ♦ Genetics ♦ Metabolism ♦ Photic Stimulation ♦ Pregnancy ♦ Rats ♦ Rats, Wistar ♦ Anatomy & Histology ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Mammalian retina contains a circadian clock that is composed of components similar to those of the master circadian clock within the suprachiasmatic nuclei of the hypothalamus. The aim of the present study was to elucidate whether, when, and where the transcripts of the clock genes Per1 and Per2 and the immediate early gene c-fos are spontaneously expressed and/or induced by light in the newborn rat retina. At postnatal day 1 (P1), P3, P5, and P10, Wistar rat pups were released into constant darkness, and a 30-minute light pulse was administered during the subjective day or during the first or second part of subjective night. Gene expression was determined 30 minutes, 1 hour, 2 hours, and 4 hours after the light pulse by in situ hybridization followed by emulsion autoradiography. Endogenous expression of Per1 was detected in the neuroblastic retina, and Per2 expression was detected in the inner part of the neuroblastic retina from birth. Light pulses induced c-fos expression in ganglion cells from P1. Until P5, the cells were localized in the dorsal part of the retina, but, at P10, they were already distributed across the entire retinal circumference. Light pulses also induced the expression of c-fos and Per1 in the retinal pigment epithelium until P3, but not afterward. Expression of the Per2 gene was not photoresponsive until P10. These data demonstrate that the rat retina is light-sensitive immediately after birth. During early postnatal development, the spatial distribution of spontaneous and light-induced gene expression within the retinal layers changes gradually.
Description Country affiliation: Czech Republic
Author Affiliation: Mateju K ( Department of Neurohumoral Regulations, Institute of Physiology, Academy of Sciences of the Czech Republic v.v.i., Prague 14220, Czech Republic.)
ISSN 00219967
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-09-01
Publisher Place United States
e-ISSN 10969861
Journal Journal of Comparative Neurology
Volume Number 518
Issue Number 17


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Source: WHO-Global Index Medicus