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Author Goo, Bon Geun ♦ Park, Jae Kweon
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Elsevier
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Chemical engineering
Subject Domain (in MeSH) Bacteria ♦ Organisms ♦ Macromolecular Substances ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Physical Phenomena ♦ Chemical Phenomena ♦ Biological Sciences
Subject Keyword Discipline Biomedical Engineering ♦ Discipline Microbiology ♦ Bacillus Cereus ♦ Enzymology ♦ Bacterial Proteins ♦ Chemistry ♦ Glycoside Hydrolases ♦ Biosynthesis ♦ Isolation & Purification ♦ Carbohydrate Conformation ♦ Chitosan ♦ Hydrogen-ion Concentration ♦ Hydrolysis ♦ Kinetics ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract An alkalophilic extracellular chitosanase (ACTase) was characterized from the culture supernatant of Bacillus cereus GU-02. Kinetic properties of ACTase produced from B. cereus GU-02 after cultivation in anaerobic condition, alkaline medium (pH 10) at 37°C for 3 days were investigated. ACTase was found to be stable in alkaline pH range from 8 to 10. Interestingly, optimum pH and temperature were estimated to be 10 and 37°C, respectively, where ACTase showed chitosan degrading activity (87%), which was enhanced by 15% in the presence of calcium ions (8 mM). The ACTase produced from B. cereus GU-02 was partially purified from the culture supernatant, and its enzymatic activity was kinetically characterized. The Vmax and Km were estimated with a chitosan (degree of deacetylation, DD 92% as substrate) as 0.038 U/min/µg protein and 0.327 µM, respectively. A combination of the TLC and MALDI-TOF MS results showed that the chitosan oligosaccharides obtained from the hydrolysis of high molecular weight chitosan (HMWC) by ACTase of the B. cereus GU-2 comprise oligomers with degree of polymerization (DP) mainly from dimers to pentamers. High production of ACTase and chitooligosaccharides may be useful for various industrial and biological applications.
Description Author Affiliation: Goo BG ( Department of Pharmaceutical Science, Gachon University, 191 Hambakmoeiro, Yeonsu-dong, Yeonsu-gu, InCheon 406-799, Republic of Korea.); Park JK ( Department of Life Science, College of Bionano, Gachon University, 191 Hambakmoeiro, Yeonsu-dong, Yeonsu-gu, InCheon 406-799, Republic of Korea. Electronic address: jkpark@gachon.ac.kr.)
ISSN 13891723
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2014-06-01
Publisher Place Japan
e-ISSN 13474421
Journal Journal of Bioscience and Bioengineering
Volume Number 117
Issue Number 6


Source: WHO-Global Index Medicus