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Author Lazova, Rossitza ♦ Klump, Vincent ♦ Pawelek, John
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Neoplasms ♦ Diseases ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Diagnosis ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Cell Physiological Phenomena ♦ Biological Sciences ♦ Persons ♦ Persons
Subject Keyword Discipline Pathology ♦ Discipline Dermatology ♦ Autophagy ♦ Physiology ♦ Melanoma ♦ Physiopathology ♦ Skin Neoplasms ♦ Adult ♦ Aged ♦ Aged, 80 And Over ♦ Endoplasmic Reticulum ♦ Metabolism ♦ Pathology ♦ Female ♦ Humans ♦ Immunohistochemistry ♦ Male ♦ Mannose-binding Lectins ♦ Melanins ♦ Membrane Proteins ♦ Microscopy, Electron ♦ Middle Aged ♦ Phagosomes ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract We show that malignant melanoma cells display high levels of autophagy, a cytoplasmic process of protein and organelle digestion that provides an energy source in times of nutrient deprivation. In a panel of 12 cases of cutaneous malignant melanoma of the superficial spreading type, cells in florid melanoma in situ (MIS) and invasive cells in the dermis appeared to be undergoing autophagy. Autophagosomes were detected through immunohistochemistry using the marker LC3B (microtubule-associated light chain 3B), and by electron microscopy. Some autophagosomes contained melanized melanosomes, accounting for the phenomenon of 'coarse melanin' in malignant melanoma. Autophagosomes also contained the Golgi 58k protein, a structural component of the Golgi apparatus, and beta1,6-branched oligosaccharides, indicating that at least some of the autophagosomal proteins were glycosylated with these structures. The findings suggest that autophagy could be a constitutive metabolic state for invasive and metastatic melanoma cells. Interestingly, a similar phenotype was also expressed by tumor-associated melanophages. The findings are consistent with previous reports that endoplasmic reticulum (ER) stress drives melanoma progression, since ER stress is known to trigger autophagy. The results suggest that therapies inhibiting autophagy may be effective for the treatment of malignant melanoma by depriving cells of an important energy source.
Description Country affiliation: United States
Author Affiliation: Lazova R ( Department of Dermatology, Yale University School of Medicine, New Haven, CT 06520-8059, USA.)
ISSN 03036987
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-02-01
Publisher Place United States
e-ISSN 16000560
Journal Journal of Cutaneous Pathology
Volume Number 37
Issue Number 2

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Source: WHO-Global Index Medicus