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Author Yokoyama, Mari ♦ Miwa, Hiroto ♦ Maeda, Satoshi ♦ Wakitani, Shigeyuki ♦ Takagi, Mutsumi
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Elsevier
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Chemical engineering
Subject Domain (in MeSH) Cells ♦ Fluids and Secretions ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Cell Physiological Phenomena ♦ Physiological Phenomena ♦ Biological Sciences ♦ Persons ♦ Persons
Subject Keyword Discipline Biomedical Engineering ♦ Discipline Microbiology ♦ Chondrocytes ♦ Cytology ♦ Physiology ♦ Chondrogenesis ♦ Mesenchymal Stromal Cells ♦ Serum ♦ Metabolism ♦ Aged ♦ Cell Differentiation ♦ Cell Proliferation ♦ Cells, Cultured ♦ Humans ♦ Male ♦ Journal Article
Abstract Mesenchymal stem cells (MSCs) should be expanded in vitro while maintaining their multilineage potential before differentiation to one mesenchymal lineage for application to regeneration therapy. The effect of fetal calf serum (FCS) on undesirable differentiation during subcultivations for the expansion was investigated. The expression level of the aggrecan gene, which is a marker of chondrogenic differentiation, gradually and markedly increased during the subcultivations of MSCs with the addition of 10% FCS and without additional cytokines. The percentage of cells positive for CD90 and CD166, which are markers of MSCs, decreased, and the percentage of large polygonal cells and the average cell adhesion area increased during the expansion. There was a marked difference in the increase in the aggrecan expression level between the two expansion cultures employing different FCS lots, although their proliferation rates were almost the same. The decrease in FCS concentration resulted in a higher percentage of CD90(+)CD166(+) cells, a lower percentage of large polygonal cells, and a lower level of aggrecan expression. Consequently, FCS components could stimulate MSC differentiation to chondrocytes and a lower concentration could decrease this differentiation.
Description Country affiliation: Japan
Author Affiliation: Yokoyama M ( Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, Kita-ku N13W8, Sapporo 060-8628, Japan.)
ISSN 13891723
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2008-07-01
Publisher Place Japan
e-ISSN 13474421
Journal Journal of Bioscience and Bioengineering
Volume Number 106
Issue Number 1


Source: WHO-Global Index Medicus