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Author Nakagawa, Atsushi ♦ Suzuki, Takahiro ♦ Kato, Ko ♦ Shinmyo, Atsuhiko ♦ Suzuki, Toshio
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Elsevier
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Biochemistry ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Pharmacology and therapeutics ♦ Chemical engineering
Subject Domain (in MeSH) Bacteria ♦ Organisms ♦ Organic Chemicals ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Natural Science Disciplines ♦ Physical Sciences
Subject Keyword Discipline Biomedical Engineering ♦ Discipline Microbiology ♦ Bacterial Proteins ♦ Chemistry ♦ Carboxylic Ester Hydrolases ♦ Hydroxybutyrates ♦ Chemical Synthesis ♦ Rhizobium ♦ Enzymology ♦ Biosynthesis ♦ Genetics ♦ Isolation & Purification ♦ Escherichia Coli ♦ Recombinant Proteins ♦ Soil Microbiology ♦ Journal Article
Abstract (S)-4-Chloro-3-hydroxybutyrate (CHB) is essential for the synthesis of biologically and pharmacologically important compounds. Rhizobium sp. DS-S-51 isolated from soil samples showed hydrolytic activity toward (R)-CHB in the racemate to (R)-3-hydroxy-gamma-butyrolactone (HL) under a simple composition of the reaction. Residual (S)-CHB was obtained with high optical purity. The gene encoding the enzyme concerned, designated CHB hydrolase, was isolated from DS-S-51, and the gene was highly expressed in Escherichia coli JM109. When the resolution of racemic methyl CHB (CHBM) as a substrate was performed using this recombinant cell, JM109 (pKK-R1), the hydrolytic activity was found to be 40-fold greater than that of DS-S-51, and the maximum concentration of the substrate added increased 2-fold. Moreover, (R)-HL was also obtained without decreasing the optical purity compared with that when (R)-CHBM was used as a substrate.
Description Country affiliation: Japan
Author Affiliation: Nakagawa A ( Research Laboratories of DAISO Co., Ltd., 9 Otakasu-cho, Amagasaki, Hyogo 660-0842, Japan.)
ISSN 13891723
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2008-04-01
Publisher Place Japan
e-ISSN 13474421
Journal Journal of Bioscience and Bioengineering
Volume Number 105
Issue Number 4


Source: WHO-Global Index Medicus