Thumbnail
Access Restriction
Open

Author Murphy-Holland, K. ♦ Eveleigh, D. E.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY ♦ CELLULASE ♦ QUANTITATIVE CHEMICAL ANALYSIS ♦ ACRYLAMIDE ♦ ELECTROPHORESIS ♦ GELS ♦ POLYMERS ♦ TRICHODERMA VIRIDE ♦ AMIDES ♦ CHEMICAL ANALYSIS ♦ COLLOIDS ♦ DISPERSIONS ♦ ENZYMES ♦ FUNGI ♦ GLYCOSYL HYDROLASES ♦ HYDROLASES ♦ O-GLYCOSYL HYDROLASES ♦ ORGANIC COMPOUNDS ♦ ORGANIC NITROGEN COMPOUNDS ♦ PLANTS ♦ TRICHODERMA ♦ Chemical & Spectral Procedures
Abstract Endoglucanase and exoglucanase components of cellulase can be detected and differentiated after polyacrylamide gel electrophoresis by performing activity stains. Endoglucanase activity was visualized in carboxymethyl cellulose agar replicas of gels by staining with Congo red. General ..beta..-1,4-glucanase activity was located by soaking the gel in a solution of NaBH/sub 4/-reduced cellulo-oligosaccharides, and detecting the formation of reducing sugars by reaction with triphenyl tetrazolium chloride. Endoglucanases are active in both assays, while exoglucanases can be distinguished by their activity in the cellulo-oligosaccharide assay only. This methodology has facilitated the purification and characterization of cellulase components from Trichoderma reesei and Microbispora bispora.
Educational Use Research
Learning Resource Type Article
Publisher Date 1984-01-01
Publisher Place United States
Journal Anal. Biochem.
Volume Number 140
Organization Rutgers Univ., New Brunswick, NJ


Open content in new tab

   Open content in new tab