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Author Zhang, Yupeng ♦ He, Jing ♦ Zhao, Jing ♦ Xu, Min ♦ Lou, Danwen ♦ Tso, Patrick ♦ Li, Zongfang ♦ Li, Xiaoming
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ DISEASES ♦ GENES ♦ LIVER CELLS ♦ RECEPTORS ♦ SERINE PROTEINASES ♦ TIME DEPENDENCE ♦ VISIBLE RADIATION
Abstract ApoA4 exerts anti-inflammatory effects, but the mechanism remains unclear. SERPINA3 is a member of the serine proteinase inhibitor gene family, and has been shown to be involved in anti-inflammation and associated with a number of human diseases. In this study, we revealed that ApoA4 stimulates the gene expression of SERPINA3 in mouse hepatocytes both in vivo and in vitro, in a dose- and time-dependent manner. The transcriptional response of SERPINA3 to ApoA4 is regulated through the binding of ApoA4 with nuclear receptors NR4A1 and NR1D1 on the SERPINA3 promoter, which was verified with ChIP, Luciferase activity assay and RNA interference-mediated NR4A1 or NR1D1 gene knockdown. These data suggests that ApoA4 transcriptionally induced SERPINA3 expression via NR1D1 and NR4A1. Our findings may throw light on the function of ApoA4 in inflammatory responses and acute-phase reactions, as well as the development of SERPINA3 relative diseases. - Highlights: • ApoA4 stimulates the expression of SERPINA3 in hepatocytes. • The mechanism works through ApoA4 on the transcription of SERPINA3 via NR4A1 and NR1D1. • ApoA4 may act as an anti-inflammatory response in SERPINA3 relative diseases.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2017-05-27
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 487
Issue Number 2


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