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Author Viljoen, A. J. ♦ Tappel, A. L.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT. ♦ BASIC BIOLOGICAL SCIENCES ♦ PROTEINS ♦ FRACTIONATION ♦ SELENIUM ♦ METABOLISM ♦ KIDNEYS ♦ LIQUID COLUMN CHROMATOGRAPHY ♦ METHIONINE ♦ MOLECULAR WEIGHT ♦ PEROXIDASES ♦ RATS ♦ SELENIUM 75 ♦ TRACER TECHNIQUES ♦ TRYPSIN ♦ AMINO ACIDS ♦ ANIMALS ♦ BETA DECAY RADIOISOTOPES ♦ BODY ♦ CARBOXYLIC ACIDS ♦ CHROMATOGRAPHY ♦ DAYS LIVING RADIOISOTOPES ♦ DRUGS ♦ ELECTRON CAPTURE RADIOISOTOPES ♦ ELEMENTS ♦ ENZYMES ♦ EVEN-ODD NUCLEI ♦ HYDROLASES ♦ INTERMEDIATE MASS NUCLEI ♦ ISOTOPE APPLICATIONS ♦ ISOTOPES ♦ LIPOTROPIC FACTORS ♦ MAMMALS ♦ NUCLEI ♦ ORGANIC ACIDS ♦ ORGANIC COMPOUNDS ♦ ORGANIC SULFUR COMPOUNDS ♦ ORGANS ♦ OXIDOREDUCTASES ♦ PEPTIDE HYDROLASES ♦ RADIOISOTOPES ♦ RODENTS ♦ SELENIUM ISOTOPES ♦ SEMIMETALS ♦ SEPARATION PROCESSES ♦ SERINE PROTEINASES ♦ VERTEBRATES ♦ Chemicals Metabolism & Toxicology ♦ Biochemistry- Tracer Techniques
Abstract Rat kidney selenium (Se)-containing proteins were studied by isotopic labeling with (/sup 75/Se)selenite or (/sup 75/Se)selenomethionine via three routes: oral, intraperitoneal injection, and incubation of kidney slices with the isotope. The two major Se-containing proteins in kidney were fractionated and partially characterized. /sup 75/Se elution profiles from Sephadex G-150 chromatography were similar for each labeling protocol, except for the profile obtained following incubation of slices with (/sup 75/Se)selenomethionine. Of the two major /sup 75/Se-containing proteins, the one eluting at the void volume during Sephadex G-150 fractionation had a subunit of 23,000 Mr. The /sup 75/Se-labeled tryptic peptide from this protein and a /sup 75/Se-containing tryptic peptide from glutathione peroxidase had the same elution time from an HPLC column. A 75,000 Mr /sup 75/Se-containing protein had a 65,000 Mr subunit, and the /sup 75/Se-labeled tryptic peptide from this protein eluted from the HPLC column before that of glutathione peroxidase. Glutathione peroxidase is the most abundant kidney selenoprotein. Injection of animals with /sup 75/Se is the method of choice for isotopic labeling of rat kidney Se-containing proteins. Appropriate methods were developed that can be used in future studies of kidney Se-containing proteins.
Educational Use Research
Learning Resource Type Article
Publisher Date 1988-08-01
Publisher Place United States
Journal J. Inorg. Biochem.
Volume Number 33
Issue Number 4
Organization Univ. of California, Davis (USA)


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