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Author Aloraefy, Mamdouh ♦ Pfefer, T. Joshua ♦ Ramella-Roman, Jessica C. ♦ Sapsford, Kim E.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Multidisciplinary Digital Publishing Institute
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Biochemistry ♦ Technology ♦ Medicine & health ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Carbohydrates ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment
Subject Keyword Discipline Biotechnology ♦ Biosensing Techniques ♦ Instrumentation ♦ Concanavalin A ♦ Chemistry ♦ Fluorescence Resonance Energy Transfer ♦ Glucose ♦ Analysis ♦ Equipment Design ♦ Equipment Failure Analysis ♦ In Vitro Techniques ♦ Reproducibility Of Results ♦ Sensitivity And Specificity ♦ Comparative Study ♦ Evaluation Studies ♦ Journal Article ♦ Validation Studies
Abstract Rapid, accurate, and minimally-invasive glucose biosensors based on Förster Resonance Energy Transfer (FRET) for glucose measurement have the potential to enhance diabetes control. However, a standard set of in vitro approaches for evaluating optical glucose biosensor response under controlled conditions would facilitate technological innovation and clinical translation. Towards this end, we have identified key characteristics and response test methods, fabricated FRET-based glucose biosensors, and characterized biosensor performance using these test methods. The biosensors were based on competitive binding between dextran and glucose to concanavalin A and incorporated long-wavelength fluorescence dye pairs. Testing characteristics included spectral response, linearity, sensitivity, limit of detection, kinetic response, reversibility, stability, precision, and accuracy. The biosensor demonstrated a fluorescence change of 45% in the presence of 400 mg/dL glucose, a mean absolute relative difference of less than 11%, a limit of detection of 25 mg/dL, a response time of 15 min, and a decay in fluorescence intensity of 72% over 30 days. The battery of tests presented here for objective, quantitative in vitro evaluation of FRET glucose biosensors performance have the potential to form the basis of future consensus standards. By implementing these test methods for a long-visible-wavelength biosensor, we were able to demonstrate strengths and weaknesses with a new level of thoroughness and rigor.
Description Country affiliation: United States
Author Affiliation: Aloraefy M ( Center for Devices and Radiological Health, Food and Drug Administration, Silver Spring, MD 20993, USA. Mamdouh.Aloraefy@fda.hhs.gov.); Pfefer TJ ( Center for Devices and Radiological Health, Food and Drug Administration, Silver Spring, MD 20993, USA. Joshua.Pfefer@fda.hhs.gov.); Ramella-Roman JC ( Department of Biomedical Engineering and Herbert Wertheim College of Medicine, Florida International University, Miami, FL 33174, USA. jramella@fiu.edu.); Sapsford KE ( Center for Devices and Radiological Health, Food and Drug Administration, Silver Spring, MD 20993, USA. Kim.Sapsford@fda.hhs.gov.)
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2014-07-08
Publisher Place Switzerland
e-ISSN 14248220
Journal Sensors
Volume Number 14
Issue Number 7


Source: WHO-Global Index Medicus