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Author Soler, Maria ♦ Estevez, M-Carmen ♦ Alvarez, Mar ♦ Otte, Marinus A. ♦ Sepulveda, Borja ♦ Lechuga, Laura M.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Multidisciplinary Digital Publishing Institute
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Agriculture & related technologies ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Fluids and Secretions ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Inorganic Chemicals ♦ Organic Chemicals ♦ Hormones, Hormone Substitutes, and Hormone Antagonists ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Technology, Industry, and Agriculture ♦ Technology and Food and Beverages
Subject Keyword Discipline Biotechnology ♦ Antibodies ♦ Immunology ♦ Biological Markers ♦ Analysis ♦ Body Fluids ♦ Metabolism ♦ Immunoassay ♦ Methods ♦ Nanostructures ♦ Chemistry ♦ Antibodies, Immobilized ♦ Bacterial Proteins ♦ Blood ♦ Urine ♦ Biosensing Techniques ♦ Instrumentation ♦ C-reactive Protein ♦ Chorionic Gonadotropin ♦ Gold ♦ Humans ♦ Polyethylene Glycols ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Design of an optimal surface biofunctionalization still remains an important challenge for the application of biosensors in clinical practice and therapeutic follow-up. Optical biosensors offer real-time monitoring and highly sensitive label-free analysis, along with great potential to be transferred to portable devices. When applied in direct immunoassays, their analytical features depend strongly on the antibody immobilization strategy. A strategy for correct immobilization of antibodies based on the use of ProLinker™ has been evaluated and optimized in terms of sensitivity, selectivity, stability and reproducibility. Special effort has been focused on avoiding antibody manipulation, preventing nonspecific adsorption and obtaining a robust biosurface with regeneration capabilities. ProLinker™-based approach has demonstrated to fulfill those crucial requirements and, in combination with PEG-derivative compounds, has shown encouraging results for direct detection in biological fluids, such as pure urine or diluted serum. Furthermore, we have implemented the ProLinker™ strategy to a novel nanoplasmonic-based biosensor resulting in promising advantages for its application in clinical and biomedical diagnosis.
Description Country affiliation: Spain
Author Affiliation: Soler M ( Nanobiosensors and Bioanalytical Applications Group, Institut Català de Nanociència i Nanotecnologia (ICN2), CSIC & CIBER-BBN, Bellaterra, Barcelona 08193, Spain. maria.soler@cin2.es.); Estevez MC ( Nanobiosensors and Bioanalytical Applications Group, Institut Català de Nanociència i Nanotecnologia (ICN2), CSIC & CIBER-BBN, Bellaterra, Barcelona 08193, Spain. mcarmen.estevez@cin2.es.); Alvarez M ( Nanobiosensors and Bioanalytical Applications Group, Institut Català de Nanociència i Nanotecnologia (ICN2), CSIC & CIBER-BBN, Bellaterra, Barcelona 08193, Spain. mar.alvarez@cin2.es.); Otte MA ( Nanobiosensors and Bioanalytical Applications Group, Institut Català de Nanociència i Nanotecnologia (ICN2), CSIC & CIBER-BBN, Bellaterra, Barcelona 08193, Spain. bert.otte@cin2.es.); Sepulveda B ( Nanobiosensors and Bioanalytical Applications Group, Institut Català de Nanociència i Nanotecnologia (ICN2), CSIC & CIBER-BBN, Bellaterra, Barcelona 08193, Spain. borja.sepulveda@cin2.es.); Lechuga LM ( Nanobiosensors and Bioanalytical Applications Group, Institut Català de Nanociència i Nanotecnologia (ICN2), CSIC & CIBER-BBN, Bellaterra, Barcelona 08193, Spain. laura.lechuga@cin2.es.)
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2014-01-29
Publisher Place Switzerland
e-ISSN 14248220
Journal Sensors
Volume Number 14
Issue Number 2


Source: WHO-Global Index Medicus