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Author Díaz, Pedro ♦ Betti, Marco ♦ Sánchez, Diego H. ♦ Udvardi, Michael K. ♦ Monza, Jorge ♦ Márquez, Antonio J.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Cells ♦ Plant Structures ♦ Anatomy ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physical Phenomena ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Physiological Phenomena ♦ Biological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Botany ♦ Droughts ♦ Gene Expression Profiling ♦ Glutamate-ammonia Ligase ♦ Deficiency ♦ Genetics ♦ Metabolism ♦ Plastids ♦ Enzymology ♦ Proline ♦ Gene Expression Regulation, Plant ♦ Genes, Plant ♦ Kinetics ♦ Mutation ♦ Oligonucleotide Array Sequence Analysis ♦ Plant Leaves ♦ Reverse Transcriptase Polymerase Chain Reaction ♦ Stress, Physiological ♦ Transcription, Genetic ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract The role of plastidic glutamine synthetase (GS2) in proline biosynthesis and drought stress responses in Lotus japonicus was investigated using the GS2 mutant, Ljgln2-2. Wild-type (WT) and mutant plants were submitted to different lengths of time of water and nutrient solution deprivation. Several biochemical markers were measured and the transcriptional response to drought was determined by both quantitative real-time polymerase chain reaction and transcriptomics. The Ljgln2-2 mutant exhibited normal sensitivity to mild water deprivation, but physiological, biochemical and massive transcriptional differences were detected in the mutant, which compromised recovery (rehydration) following re-watering after severe drought stress. Proline accumulation during drought was substantially lower in mutant than in WT plants, and significant differences in the pattern of expression of the genes involved in proline metabolism were observed. Transcriptomic analysis revealed that about three times as many genes were regulated in response to drought in Ljgln2-2 plants compared with WT. The transcriptomic and accompanying biochemical data indicate that the Ljgln2-2 mutant is subject to more intense cellular stress than WT during drought. The results presented here implicate plastidic GS2 in proline production during stress and provide interesting insights into the function of proline in response to drought.
Description Country affiliation: Spain
Author Affiliation: Díaz P ( Departamento de Bioquímica Vegetal y Biología Molecular, Facultad de Química, Universidad de Sevilla, Calle Profesor García González 1, 41012-Sevilla, Spain.)
ISSN 0028646X
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-12-01
Publisher Place Great Britain (UK)
e-ISSN 14698137
Journal New Phytologist
Volume Number 188
Issue Number 4


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Source: WHO-Global Index Medicus