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Author Singh, Harpal ♦ Shimojima, Masayuki ♦ Shiratori, Tomomi ♦ An, Le Van ♦ Sugamata, Masami ♦ Yang, Ming
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Multidisciplinary Digital Publishing Institute
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Agriculture & related technologies ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Eukaryota ♦ Organisms ♦ Bacterial Infections and Mycoses ♦ Diseases ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Biological Sciences ♦ Technology, Industry, and Agriculture ♦ Technology and Food and Beverages
Subject Keyword Discipline Biotechnology ♦ Communicable Diseases ♦ Diagnosis ♦ Enzyme-linked Immunosorbent Assay ♦ Standards ♦ Printing, Three-dimensional ♦ Immunology ♦ Humans ♦ Immunoglobulin G ♦ Analysis ♦ Sensitivity And Specificity ♦ Surface Properties ♦ Journal Article ♦ Research Support, Non-u.s. Gov't ♦ Validation Studies
Abstract Enzyme-linked Immunosorbent Assay (ELISA)-based diagnosis is the mainstay for measuring antibody response in infectious diseases and to support pathogen identification of potential use in infectious disease outbreaks and clinical care of individual patients. The development of laboratory diagnostics using readily available 3D printing technologies provides a timely opportunity for further expansion of this technology into immunodetection systems. Utilizing available 3D printing platforms, a '3D well' was designed and developed to have an increased surface area compared to those of 96-well plates. The ease and rapidity of the development of the 3D well prototype provided an opportunity for its rapid validation through the diagnostic performance of ELISA in infectious disease without modifying current laboratory practices for ELISA. The improved sensitivity of the 3D well of up to 2.25-fold higher compared to the 96-well ELISA provides a potential for the expansion of this technology towards miniaturization and Lab-On-a-Chip platforms to reduce time, volume of reagents and samples needed for such assays in the laboratory diagnosis of infectious and other diseases including applications in other disciplines.
Description Country affiliation: Japan
Author Affiliation: Singh H ( Department of Intelligent Mechanical Systems, Graduate School of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo 191-0065, Japan. singhha3@gmail.com.); Shimojima M ( Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan. singhha3@gmail.com.); Shiratori T ( Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan. shimoji-@nih.go.jp.); An le V ( Department of Intelligent Mechanical Systems, Graduate School of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo 191-0065, Japan. tom7032@gmail.com.); Sugamata M ( Department of Microbiology, Hue University of Medicine and Pharmacy, 6 Ngo Quyen St., Hue 47000, Vietnam. levanan.hump@gmail.com.); Yang M ( Department of Microbiology, Hue University of Medicine and Pharmacy, 6 Ngo Quyen St., Hue 47000, Vietnam. bmsasugatmu@gmail.com.)
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2015-07-08
Publisher Place Switzerland
e-ISSN 14248220
Journal Sensors
Volume Number 15
Issue Number 7


Source: WHO-Global Index Medicus