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Author Bakthavatchalu, Sasirekha ♦ Shakthivel, Uma ♦ Mishra, Tannu
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher African Field Epidemiology Network
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Manufacture for specific uses ♦ Precision instruments & other devices
Subject Domain (in MeSH) Fluids and Secretions ♦ Anatomy ♦ Eukaryota ♦ Bacteria ♦ Organisms ♦ Bacterial Infections and Mycoses ♦ Diseases ♦ Inorganic Chemicals ♦ Organic Chemicals ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Diagnosis ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Microbiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Medicine ♦ Bacterial Proteins ♦ Analysis ♦ Boronic Acids ♦ Pharmacology ♦ Disk Diffusion Antimicrobial Tests ♦ Enterobacteriaceae ♦ Enzymology ♦ Beta-lactam Resistance ♦ Beta-lactamases ♦ Anti-bacterial Agents ♦ Antagonists & Inhibitors ♦ Biosynthesis ♦ Genetics ♦ Body Fluids ♦ Microbiology ♦ Cefoxitin ♦ Metabolism ♦ Cephalosporin Resistance ♦ Cephalosporins ♦ Clavulanic Acid ♦ Drug Effects ♦ Isolation & Purification ♦ Enterobacteriaceae Infections ♦ Enzyme Induction ♦ Gene Expression Regulation, Bacterial ♦ Humans ♦ Substrate Specificity ♦ Beta-lactamase Inhibitors ♦ Comparative Study ♦ Journal Article ♦ Retracted Publication
Abstract INTRODUCTION: The occurrence of multiple ß-lactamases among bacteria only limits the therapeutic options but also poses a challenge. A study using boronic acid (BA), an AmpC enzyme inhibitor, was designed to detect the combined expression of AmpC ß-lactamases and extended-spectrum ß-lactamases (ESBLs) in bacterial isolates further different phenotypic methods are compared to detect ESBL and AmpC. METHODS: A total of 259 clinical isolates of Enterobacteriaceae were isolated and screened for ESBL production by (i) CLSI double-disk diffusion method (ii) cefepime- clavulanic acid method (iii) boronic disk potentiation method. AmpC production was detected using cefoxitin alone and in combination with boronic acid and confirmation was done by three dimensional disk methods. Isolates were also subjected to detailed antibiotic susceptibility test. RESULTS: Among 259 isolates, 20.46% were coproducers of ESBL and AmpC, 26.45% were ESBL and 5.40% were AmpC. All of the 53 AmpC and ESBL coproducers were accurately detected by boronic acid disk potentiation method. CONCLUSION: The BA disk test using Clinical and Laboratory Standards Institute methodology is simple and very efficient method that accurately detects the isolates that harbor both AmpCs and ESBLs.
Description Country affiliation: India
Author Affiliation: Bakthavatchalu S ( Department of Microbiology, Centre for Post Graduate Studies, Jain University, Bangalore, Karnataka--560 011, India.)
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2013-01-01
Publisher Place Uganda
e-ISSN 19378688
Journal Pan African Medical Journal
Volume Number 14


Source: WHO-Global Index Medicus