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Author Huang, Shih-Hao ♦ Yu, Chu-Hung ♦ Chien, Yi-Lung
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Multidisciplinary Digital Publishing Institute
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics
Subject Domain (in MeSH) Animal Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Inorganic Chemicals ♦ Chemicals and Drugs ♦ Physical Phenomena ♦ Biological Sciences
Subject Keyword Discipline Biotechnology ♦ Embryo, Nonmammalian ♦ Metabolism ♦ Light ♦ Oxygen ♦ Animals ♦ Radiation Effects ♦ Zebrafish ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract We have developed a digital light modulation system that utilizes a modified commercial projector equipped with a laser diode as a light source for quantitative measurements of in vivo tissue oxygenation in an unanesthetized zebrafish embryo via phase-based phosphorescence lifetime detection. The oxygen-sensitive phosphorescent probe (Oxyphor G4) was first inoculated into the bloodstream of 48 h post-fertilization (48 hpf) zebrafish embryos via the circulation valley to rapidly disperse probes throughout the embryo. The unanesthetized zebrafish embryo was introduced into the microfluidic device and immobilized on its lateral side by using a pneumatically actuated membrane. By controlling the illumination pattern on the digital micromirror device in the projector, the modulated excitation light can be spatially projected to illuminate arbitrarily-shaped regions of tissue of interest for in vivo oxygen measurements. We have successfully measured in vivo oxygen changes in the cardiac region and cardinal vein of a 48 hpf zebrafish embryo that experience hypoxia and subsequent normoxic conditions. Our proposed platform provides the potential for the real-time investigation of oxygen distribution in tissue microvasculature that relates to physiological stimulation and diseases in a developing organism.
Description Country affiliation: Taiwan
Author Affiliation: Huang SH ( Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung 202-24, Taiwan.; Yu CH ( Center for Marine Mechatronic Systems, CMMS, National Taiwan Ocean University, Keelung 202-24, Taiwan.; Chien YL ( Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, Keelung 202-24, Taiwan.
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2015-04-08
Publisher Place Switzerland
e-ISSN 14248220
Journal Sensors
Volume Number 15
Issue Number 4

Source: WHO-Global Index Medicus