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Author Römer, Patrick ♦ Recht, Sabine ♦ Strauss, Tina ♦ Elsaesser, Janett ♦ Schornack, Sebastian ♦ Boch, Jens ♦ Wang, Shiping ♦ Lahaye, Thomas
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Human physiology ♦ Pharmacology and therapeutics
Subject Domain (in MeSH) Bacteria ♦ Organisms ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Microbiological Phenomena ♦ Plant Physiological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Botany ♦ Bacterial Proteins ♦ Metabolism ♦ Gene Expression Regulation, Plant ♦ Genes, Plant ♦ Oryza Sativa ♦ Genetics ♦ Plant Diseases ♦ Microbiology ♦ Xanthomonas ♦ Pathogenicity ♦ Amino Acids ♦ Glucuronidase ♦ Host-pathogen Interactions ♦ Promoter Regions, Genetic ♦ Physiology ♦ Transcription Factors ♦ Transcriptional Activation ♦ Up-regulation ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract *Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues of each repeat define specific pairing to UPT boxes. *Here we employed the TALe code to predict potential UPT boxes in TALe-induced host promoters and analyzed these via beta-glucuronidase (GUS) reporter and electrophoretic mobility shift assays (EMSA). *We demonstrate that the Xa13, OsTFX1 and Os11N3 promoters from rice are induced directly by the Xanthomonas oryzae pv. oryzae TALes PthXo1, PthXo6 and AvrXa7, respectively. We identified and functionally validated a UPT box in the corresponding rice target promoter for each TALe and show that box mutations suppress TALe-mediated promoter activation. Finally, EMSA demonstrate that code-predicted UPT boxes interact specifically with corresponding TALes. *Our findings show that variations in the UPT boxes of different rice accessions correlate with susceptibility or resistance of these accessions to the bacterial blight pathogen.
Description Country affiliation: Germany
Author Affiliation: Römer P ( Institute of Biology, Martin-Luther-University Halle-Wittenberg, 06099 Halle (Saale), Germany.)
ISSN 0028646X
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2010-09-01
Publisher Place Great Britain (UK)
e-ISSN 14698137
Journal New Phytologist
Volume Number 187
Issue Number 4


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Source: WHO-Global Index Medicus