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Author Mekonnen, Daniel ♦ Admassu, Aschalew ♦ Wassie, Belaynew ♦ Biadglegne, Fantahun
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher African Field Epidemiology Network
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Social sciences ♦ Social problems & services; associations ♦ Social welfare problems & services ♦ Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Natural history of organisms ♦ Microorganisms, fungi & algae ♦ Technology ♦ Medicine & health ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases ♦ Management & auxiliary services ♦ General management
Subject Domain (in MeSH) Eukaryota ♦ Bacteria ♦ Organisms ♦ Bacterial Infections and Mycoses ♦ Diseases ♦ Inorganic Chemicals ♦ Chemical Actions and Uses ♦ Chemicals and Drugs ♦ Physical Phenomena ♦ Microbiological Phenomena ♦ Biological Sciences ♦ Health Care Facilities, Manpower, and Services ♦ Health Services Administration ♦ Health Care ♦ Geographic Locations ♦ Geographic Locations
Subject Keyword Discipline Medicine ♦ Disinfectants ♦ Pharmacology ♦ Health Facilities ♦ Mycobacterium Tuberculosis ♦ Drug Effects ♦ Sodium Hypochlorite ♦ Administration & Dosage ♦ Drug Resistance, Bacterial ♦ Ethiopia ♦ Humans ♦ Practice Guidelines As Topic ♦ Time Factors ♦ Tuberculosis ♦ Prevention & Control ♦ Journal Article
Abstract INTRODUCTION: In Ethiopia, the most widely used disinfectant is 5% Hypochlorites. However, Ethiopian national health safety and infection prevention guideline recommendation on the use of bleach is not consistent and varying from 0.1%-4%. The purpose of this study was therefore to assess the effective time-concentration relationship of sodium hypochlorite against Mycobacterium tuberculosis complex isolates in the absence of any organic load. METHODS: This experimental study was conducted in Bahir Dar Regional laboratory from February-June 2013. Test suspensions of 1.5 X 10(8) CFU/ml prepared using normal saline containing 0.5% tween 80. From 5% stock, 0.1%, 0.5%, 1% and 2% bleach was prepared. A 1ml of test strain suspension and 1ml of bleach mixed and allowed to stand until the specified time achieved, neutralized by 48 ml phosphate buffer. 100µl from the diluted sediment were spread on two L-J mediums and incubated at 37°C for 8 weeks. RESULTS: When 0. 1% bleach was used for 10 min, majority 11/20 of isolates showed 3 x 10(3) CFU/ml growth (ME = 4.4) which was inefficient. However, when the time increased, the log10 reduction was acceptable, ME >5 and it was effective. The bleach solution containing 0.5% and above was effective in all respective times. In this study, there is no difference observed in the tuberculocidal activity of bleach against resistant and sensitive strains. CONCLUSION: Our study showed that in the absence of any organic load, 0.1% bleaches over 15 min and 0.5% bleaches over 10 min was found to be tuberculocidal.
Spatial Coverage Ethiopia
Description Country affiliation: Ethiopia
Author Affiliation: Mekonnen D ( Bahir Dar University, College of Medicine and Health Sciences, Department of Medical Microbiology, Immunology and Parasitology, Bahir Dar, Ethiopia.); Admassu A ( Bahir Dar Regional Health Research Laboratory Center, Department of Regional Mycobacteriology Laboratory, Bahir Dar, Ethiopia.); Wassie B ( Bahir Dar University, College of Medicine and Health Sciences, School of Public Health.); Biadglegne F ( Bahir Dar University, College of Medicine and Health Sciences, Department of Medical Microbiology, Immunology and Parasitology, Bahir Dar, Ethiopia )
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2015-01-01
Publisher Place Uganda
e-ISSN 19378688
Journal Pan African Medical Journal
Volume Number 21


Source: WHO-Global Index Medicus