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Author Guo, Ying-Hui ♦ Yu, Yue-Ping ♦ Wang, Dong ♦ Wu, Chang-Ai ♦ Yang, Guo-Dong ♦ Huang, Jin-Guang ♦ Zheng, Cheng-Chao
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics ♦ Diseases
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Nutritional and Metabolic Diseases ♦ Diseases ♦ Organic Chemicals ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Chemical Phenomena ♦ Genetic Phenomena ♦ Physiological Phenomena ♦ Plant Physiological Phenomena ♦ Biological Phenomena ♦ Biological Sciences
Subject Keyword Discipline Botany ♦ Carrier Proteins ♦ Metabolism ♦ Gene Expression ♦ Gossypium ♦ Genetics ♦ Kruppel-like Transcription Factors ♦ Plant Proteins ♦ Stress, Physiological ♦ Tobacco ♦ Zinc Fingers ♦ Cell Nucleus ♦ Dehydration ♦ Droughts ♦ Gene Expression Regulation, Plant ♦ Gene Library ♦ Genes, Plant ♦ Plant Diseases ♦ Plants, Genetically Modified ♦ Salicylic Acid ♦ Salt-tolerance ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract * Zinc finger proteins are a superfamily involved in many aspects of plant growth and development. However, CCCH-type zinc finger proteins involved in plant stress tolerance are poorly understood. * A cDNA clone designated Gossypium hirsutum zinc finger protein 1 (GhZFP1), which encodes a novel CCCH-type zinc finger protein, was isolated from a salt-induced cotton (G. hirsutum) cDNA library using differential hybridization screening and further studied in transgenic tobacco Nicotiana tabacum cv. NC89. Using yeast two-hybrid screening (Y2H), proteins GZIRD21A (GhZFP1 interacting and responsive to dehydration protein 21A) and GZIPR5 (GhZFP1 interacting and pathogenesis-related protein 5), which interacted with GhZFP1, were isolated. * GhZFP1 contains two typical zinc finger motifs (Cx8Cx5Cx3H and Cx5Cx4Cx3H), a putative nuclear export sequence (NES) and a potential nuclear localization signal (NLS). Transient expression analysis using a GhZFP1::GFP fusion gene in onion epidermal cells indicated a nuclear localization for GhZFP1. RNA blot analysis showed that the GhZFP1 transcript was induced by salt (NaCl), drought and salicylic acid (SA). The regions in GhZFP1 that interact with GZIRD21A and GZIPR5 were identified using truncation mutations. * Overexpression of GhZFP1 in transgenic tobacco enhanced tolerance to salt stress and resistance to Rhizoctonia solani. Therefore, it appears that GhZFP1 might be involved as an important regulator in plant responses to abiotic and biotic stresses.
Description Country affiliation: China
Author Affiliation: Guo YH ( State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, China.)
ISSN 0028646X
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2009-01-01
Publisher Place Great Britain (UK)
e-ISSN 14698137
Journal New Phytologist
Volume Number 183
Issue Number 1


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Source: WHO-Global Index Medicus