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Author Ahkami, Amir H. ♦ Lischewski, Sandra ♦ Haensch, Klaus-T ♦ Porfirova, Svetlana ♦ Hofmann, Joerg ♦ Rolletschek, Hardy ♦ Melzer, Michael ♦ Franken, Philipp ♦ Hause, Bettina ♦ Druege, Uwe ♦ Hajirezaei, Mohammad R.
Source World Health Organization (WHO)-Global Index Medicus
Content type Text
Publisher Wiley
File Format HTM / HTML
Language English
Difficulty Level Medium
Subject Domain (in DDC) Natural sciences & mathematics ♦ Chemistry & allied sciences ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Genetics and evolution ♦ Natural history of organisms ♦ Technology ♦ Medicine & health ♦ Human anatomy, cytology, histology ♦ Human physiology ♦ Pharmacology and therapeutics
Subject Domain (in MeSH) Plant Structures ♦ Anatomy ♦ Eukaryota ♦ Organisms ♦ Organic Chemicals ♦ Lipids ♦ Amino Acids, Peptides, and Proteins ♦ Nucleic Acids, Nucleotides, and Nucleosides ♦ Chemicals and Drugs ♦ Chemical Phenomena ♦ Metabolism ♦ Genetic Phenomena ♦ Biological Sciences
Subject Keyword Discipline Botany ♦ Petunia ♦ Metabolism ♦ Plant Roots ♦ Amino Acids ♦ Carbohydrate Metabolism ♦ Genetics ♦ Cell Respiration ♦ Citric Acid Cycle ♦ Cyclopentanes ♦ Fatty Acids, Unsaturated ♦ Gene Expression Regulation, Plant ♦ Genes, Plant ♦ Glycolysis ♦ Oxylipins ♦ Cytology ♦ Enzymology ♦ Plant Proteins ♦ Rna, Messenger ♦ Journal Article ♦ Research Support, Non-u.s. Gov't
Abstract Adventitious root formation (ARF) in the model plant Petunia hybrida cv. Mitchell has been analysed in terms of anatomy, gene expression, enzymatic activities and levels of metabolites. This study focuses on the involvement of wound response and primary metabolism. Microscopic techniques were complemented with targeted transcript, enzyme and metabolite profiling using real time polymerase chain reaction (PCR), Northern blot, enzymatic assays, chromatography and mass spectrometry. Three days after severance from the stock plants, first meristematic cells appeared which further developed into root primordia and finally adventitious roots. Excision of cuttings led to a fast and transient increase in the wound-hormone jasmonic acid, followed by the expression of jasmonate-regulated genes such as cell wall invertase. Analysis of soluble and insoluble carbohydrates showed a continuous accumulation during ARF. A broad metabolite profiling revealed a strong increase in organic acids and resynthesis of essential amino acids. Substantial changes in enzyme activities and metabolite levels indicate that specific enzymes and metabolites might play a crucial role during ARF. Three metabolic phases could be defined: (i) sink establishment phase characterized by apoplastic unloading of sucrose and being probably mediated by jasmonates; (ii) recovery phase; and (iii) maintenance phase, in which a symplastic unloading occurs.
Description Country affiliation: Germany
Author Affiliation: Ahkami AH ( Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.)
ISSN 0028646X
Educational Role Student ♦ Teacher
Age Range above 22 year
Educational Use Reading ♦ Research ♦ Self Learning
Interactivity Type Expositive
Education Level UG and PG
Learning Resource Type Article
Publisher Date 2009-01-01
Publisher Place Great Britain (UK)
e-ISSN 14698137
Journal New Phytologist
Volume Number 181
Issue Number 3


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Source: WHO-Global Index Medicus