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Author Wilton, S. ♦ Dales, S.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BASIC BIOLOGICAL SCIENCES ♦ RNA POLYMERASES ♦ BIOCHEMICAL REACTION KINETICS ♦ VIRUSES ♦ INFECTIVITY ♦ ANIMAL CELLS ♦ CELL NUCLEI ♦ CYTOPLASM ♦ EFFICIENCY ♦ ENZYME ACTIVITY ♦ FIBROBLASTS ♦ FLUORESCENCE ♦ HELA CELLS ♦ IMMUNOGLOBULINS ♦ METHIONINE ♦ PATHOGENESIS ♦ RATS ♦ SULFUR 35 ♦ TRACER TECHNIQUES ♦ VIRAL DISEASES ♦ AMINO ACIDS ♦ ANIMALS ♦ BETA DECAY RADIOISOTOPES ♦ BETA-MINUS DECAY RADIOISOTOPES ♦ CARBOXYLIC ACIDS ♦ CELL CONSTITUENTS ♦ CONNECTIVE TISSUE CELLS ♦ DAYS LIVING RADIOISOTOPES ♦ DISEASES ♦ DRUGS ♦ ENZYMES ♦ EVEN-ODD NUCLEI ♦ GLOBULINS ♦ INFECTIOUS DISEASES ♦ ISOTOPE APPLICATIONS ♦ ISOTOPES ♦ KINETICS ♦ LIGHT NUCLEI ♦ LIPOTROPIC FACTORS ♦ LUMINESCENCE ♦ MAMMALS ♦ MICROORGANISMS ♦ NUCLEI ♦ NUCLEOTIDYLTRANSFERASES ♦ ORGANIC ACIDS ♦ ORGANIC COMPOUNDS ♦ ORGANIC SULFUR COMPOUNDS ♦ PARASITES ♦ PHOSPHORUS-GROUP TRANSFERASES ♦ POLYMERASES ♦ PROTEINS ♦ RADIOISOTOPES ♦ REACTION KINETICS ♦ RODENTS ♦ SOMATIC CELLS ♦ SULFUR ISOTOPES ♦ TRANSFERASES ♦ VERTEBRATES ♦ Pathology- Tracer Techniques
Abstract It is clear from previous studies that host transcriptase or RNA polymerase II (pol II) has a role in poxvirus replication. To elucidate the participation of this enzyme further, in this study the authors examined several parameters related to pol II during the cycle of vaccinia virus infection in L-strain fibroblasts, HeLa cells, and L/sub 6/H/sub 9/ rat myoblasts. Nucleocytoplasmic transposition of pol II into virus factories and virions was assessed by immunofluorescence and immunoblotting by using anti-pol II immunoglobulin G. RNA polymerase activities were compared in nuclear extracts containing cured enzyme preparations. Rates of translation into cellular or viral polypeptides were ascertained by labeling with (/sup 35/S)methionine. In L and HeLa cells, which produced vaccinia virus more abundantly, the rate of RNA polymerase and translation in controls and following infection were higher than in myoblasts. The data on synthesis and virus formation could be correlated with observations on transmigration of pol II, which was more efficient and complete in L and HeLa cells. The stimulus for pol II to leave the nucleus required the expression of both early and late viral functions. On the basis of current and past information, the authors suggest that mobilization of pol II depends on the efficiency of vaccinia virus replication and furthermore that control over vaccinia virus production by the host is related to the content or availability (or both) of pol II in different cell types.
Educational Use Research
Learning Resource Type Article
Publisher Date 1989-04-01
Publisher Place United States
Journal J. Virol.
Volume Number 63
Issue Number 4
Organization Univ. of Western Ontario, London (Canada)


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