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Author Liu, Xing ♦ Xu, Yanli ♦ Meng, Qian ♦ Zheng, Qingqing ♦ Wu, Jianhong ♦ Wang, Chen ♦ Jia, Weiping ♦ Figeys, Daniel ♦ Chang, Ying ♦ Zhou, Hu
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ANIMAL TISSUES ♦ BIOLOGICAL MARKERS ♦ BIOPSY ♦ DIAGNOSIS ♦ MUCOUS MEMBRANES ♦ NEOPLASMS ♦ PROTEINS ♦ SAMPLING ♦ VALIDATION
Abstract Colorectal cancer (CRC) is one of the most common types of malignant tumor worldwide. Currently, although many researchers have been devoting themselves in CRC studies, the process of locating biomarkers for CRC early diagnosis and prognostic is still very slow. Using a centrifugal proteomic reactor-based proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling, 2620 protein groups were quantified between cancer mucosa and adjacent normal colorectal mucosa. Of which, 403 protein groups were differentially expressed with statistic significance between cancer and normal tissues, including 195 up-regulated and 208 down-regulated proteins in cancer tissues. Three proteins (SOD3, PRELP and NGAL) were selected for further Western blot validation. And the resulting Western blot experimental results were consistent with the quantitative proteomic data. SOD3 and PRELP are down-regulated in CRC mucosa comparing to adjacent normal tissue, while NGAL is up-regulated in CRC mucosa. In conclusion, the centrifugal proteomic reactor-based label-free quantitative proteomic approach provides a highly sensitive and powerful tool for analyzing minute protein sample from tiny colorectal biopsies, which may facilitate CRC biomarkers discovery for diagnoses and prognoses. -- Highlights: •Minute amount of colonic biopsies by endoscopy is suitable for proteomic analysis. •Centrifugal proteomic reactor can be used for processing tiny clinic biopsy sample. •SOD3 and PRELP are down-regulated in CRC, while NGAL is up-regulated in CRC.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2016-08-05
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 476
Issue Number 4


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