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Author Gnetti, Letizia ♦ Madeddu, Denise ♦ Quaini, Eugenio ♦ Lorusso, Bruno ♦ Falco, Angela ♦ Ampollini, Luca ♦ Carbognani, Paolo ♦ Quaini, Federico ♦ Lagrasta, Costanza ♦ Cavalli, Stefano ♦ Gervasi, Andrea ♦ Silini, Enrico M. ♦ Frati, Caterina ♦ Rinaldi, Laura ♦ Graiani, Gallia ♦ Maselli, Davide
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher Hindawi Limited
File Format HTM / HTML
Date Created 2015-09-10
Copyright Year ©2015
Language English
Subject Domain (in LCC) R
Subject Keyword Medicine
Abstract Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs. The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays. Interestingly, these cells retain in vitro several receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation. HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy.
ISSN 23146133
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2015-01-01
e-ISSN 23146133
Journal BioMed Research International
Volume Number 2015

Source: Directory of Open Access Journals (DOAJ)