Thumbnail
Access Restriction
Open

Author Cortés-Malagón, Enoc M. ♦ Rodríguez-Uribe, Genaro ♦ Bonilla-Delgado, José ♦ Serafín-Higuera, Nicolás ♦ Damian-Morales, Gabriela ♦ Moreno-Eutimio, Mario Adán ♦ Ocadiz-Delgado, Rodolfo ♦ Lambert, Paul F. ♦ Gariglio, Patricio
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher Hindawi Limited
File Format HTM / HTML
Date Created 2016-07-26
Copyright Year ©2016
Language English
Subject Domain (in LCC) R
Subject Keyword Medicine
Abstract Objective. The aim of this study was to analyze the effects of the HPV16 E7 oncoprotein on dendritic cells (DCs) and CD11b+Gr1+ cells using the K14E7 transgenic mouse model. Materials and Methods. The morphology of DCs was analyzed in male mouse skin on epidermal sheets using immunofluorescence and confocal microscopy. Flow cytometry was used to determine the percentages of DCs and CD11b+Gr1+ cells in different tissues and to evaluate the migration of DCs. Results. In the K14E7 mouse model, the morphology of Langerhans cells and the migratory activity of dendritic cells were abnormal. An increase in CD11b+Gr1+ cells was observed in the blood and skin of K14E7 mice, and molecules related to CD11b+Gr1+ chemoattraction (MCP1 and S100A9) were upregulated. Conclusions. These data suggest that the HPV16 E7 oncoprotein impairs the function and morphology of DCs and induces the systemic accumulation of CD11b+Gr1+ cells.
ISSN 23146133
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2016-01-01
e-ISSN 23146133
Journal BioMed Research International
Volume Number 2016


Source: Directory of Open Access Journals (DOAJ)