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Author Stoker, M.
Sponsorship USDOE
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BIOLOGY AND MEDICINE ♦ CYTOLOGY ♦ INFECTIONS ♦ POLYOMA VIRUS ♦ QUANTITATIVE ANALYSIS ♦ QUANTITY RATIO ♦ RADIATION DOSES ♦ RADIATION EFFECTS ♦ RADIOSENSITIVITY ♦ SENSITIVITY ♦ SUSPENSIONS ♦ VIRUSES ♦ X RADIATION
Abstract An investigation was made on the effects of x rays on the sensitivity of BHK21 cells to transformation by polyoma virus. The x-ray sensitivity of uninfected BHK21 cells was first determined. To investigate the effect of irradiation on subsequent polyoma transformation, batches of 1-2 million BHK21 cells were exposed in suspension to various doses of irradiation. Equal numbers of cells (about one million) from each batch were infected in suspension with 10/ sup 9/ plaque-forming units of polyoma virus and assayed for transformation by the standard method. lt was found that the irradiation reduced the number of viable cells giving normal colonies more rapidly than the number giving transformed colonies. After 500 r and subsequent infection the proportion of normal viable cells had fallen from 48 to 4.4% whereas the transformed viable cells only fell from 2.5 to 0.87%. The difference led to and increase in the transformed colony frequency. It was concluded that prior irradiation of BHK21 cells followed by polyoma virus infection increases the probability that the survivors will yield transformed clones. (P.C.H.)
Educational Use Research
Learning Resource Type Article
Publisher Date 1963-11-23
Publisher Department Univ. of Glasgow
Journal Nature
Volume Number 200
Organization Univ. of Glasgow


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