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Author Tank, David C. ♦ Fant, Jeremie B. ♦ Skogen, Krissa A. ♦ Weinbergwolf, Hannah
Source Directory of Open Access Journals (DOAJ)
Content type Text
Publisher Botanical Society of America
File Format HTM / HTML
Date Created 2014-05-19
Copyright Year ©2013
Language English
Subject Domain (in LCC) Q ♦ QH301-705.5
Subject Keyword Microsatellites ♦ Hemiparasite ♦ Castilleja ♦ Biology ♦ Cross-amplification ♦ Science ♦ Castilleja sessiliflora ♦ Orobanchaceae
Abstract Premise of the study: Microsatellite primers were developed in the hemiparasitic perennial forb Castilleja sessiliflora to investigate patterns of gene flow and genetic diversity within and among populations. Methods and Results: Twelve polymorphic loci were identified in C. sessiliflora and tested on three populations (32 individuals each) sampled across the range of the species. The loci amplified di- and trinucleotide repeats with 3–14 alleles per locus. To assess cross-amplification, primer pairs were also tested on 24 additional Castilleja species that represent the morphological and geographic diversity of the genus. We provide reports of their effectiveness in all 25 taxa. Conclusions: These results indicate the utility of these primers in C. sessiliflora for future studies of genetic structure and gene flow, as well as their widespread applicability in other members of the diverse and complex genus Castilleja.
ISSN 21680450
Age Range 18 to 22 years ♦ above 22 year
Educational Use Research
Education Level UG and PG ♦ Career/Technical Study
Learning Resource Type Article
Publisher Date 2013-06-01
e-ISSN 21680450
Journal Applications in Plant Sciences
Volume Number 1
Issue Number 6
Starting Page 1200564

Source: Directory of Open Access Journals (DOAJ)