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Author Adachi, Naoki ♦ Kubota, Yoshitaka ♦ Kosaka, Kentarou ♦ Akita, Shinsuke ♦ Sasahara, Yoshitarou ♦ Kira, Tomoe ♦ Kuroda, Masayuki ♦ Mitsukawa, Nobuyuki ♦ Bujo, Hideaki ♦ Satoh, Kaneshige
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ ANGIOGENESIS ♦ ANOXIA ♦ CELL CULTURES ♦ GENES ♦ GRAFTS ♦ MESSENGER-RNA ♦ PROTEINS ♦ RADIATION DOSES ♦ STEM CELLS
Abstract Poor survival is a major problem of adipocyte transplantation. We previously reported that VEGF and MMPs secreted from transplanted adipocytes are essential for angiogenesis and adipogenesis. Pretreatment with low-dose (5 Gy) radiation (LDR) increased VEGF, MMP-2, and HIF-1 alpha mRNA expression in human ceiling culture-derived proliferative adipocytes (hccdPAs). Gene expression after LDR differed between adipose-derived stem cells (hASCs) and hccdPAs. Pretreatment with LDR improved the survival of hccdPAs under hypoxia, which is inevitable in the early stages after transplantation. Upregulation of VEGF and MMP-2 after LDR in hccdPAs is mediated by HIF-1 alpha expression. Our results suggest that pretreatment with LDR may improve adipocyte graft survival in a clinical setting through upregulation of VEGF and MMP-2 via HIF-1 alpha. - Highlights: • Ceiling culture-derived proliferative adipocytes (ccdPAs) react to radiation. • Low-dose radiation (LDR) pretreatment improves survival of ccdPAs under hypoxia. • Gene expression after LDR differs between ccdPAs and adipose-derived stem cells. • LDR-induced increase in MMP-2 and VEGF is dependent on HIF-1 alpha induction. • LDR pretreatment may improve the adipocyte graft survival rate in clinical settings.
ISSN 0006291X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-08-07
Publisher Place United States
Journal Biochemical and Biophysical Research Communications
Volume Number 463
Issue Number 4


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