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Author da Cruz, A. D. ♦ Glickman, B. W.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BIOLOGY AND MEDICINE, APPLIED STUDIES ♦ CESIUM 137 ♦ GENETIC RADIATION EFFECTS ♦ LYMPHOCYTES ♦ MUTATIONS ♦ GENETIC CONTROL ♦ MAN ♦ RADIATION DOSES ♦ PENTOSYL TRANSFERASES
Abstract The current study comprises individuals accidentally exposed to ionizing radiation of Cesium-137 after a highly radioactive radiotherapy source was removed from its protective housing and subsequently ruptured, leading to internal and external exposure of several people to significant levels of Cesium-137. Because of the mutagenic and carcinogenic effects of radiation, the genetic health of the accident victims has been studied. The genetic marker used in this study is the hprt gene and the selective assay using 6-thioguanine was performed with peripheral T-lymphocytes. Samples were drawn from 10 individuals 3.3 years after the original exposure and yielded 221 mutants from which 146 mutants were recovered for molecular analysis. From those, 110 cDNAs have been sequenced using an automated laser fluorescent DNA sequencer. The results indicate that the mutants are comprised of 46% base substitution, 12% frameshifts, 24% exon skipping and 18% of other mutations including insertions, duplications, deletions, etc. The cDNA molecular analysis based on TCR showed that only 2% of the mutants were clonally related. Out of the 36 genomic DNAs studied, 7% involved the total deletion of the hprt gene, 11% partial deletion, while 5.5% retained all the exons, but produced no cDNA. There was only 0.7% clonality as detected by TCR analysis. The nature of mutations recovered in vivo provides more detailed insights into the spectrum of mutations for the hprt gene. Such data will give us a better idea of how exposure to environmental mutagens, such as radiation, affects human populations and their health.
ISSN 08936692
Educational Use Research
Learning Resource Type Article
Publisher Date 1995-11-01
Publisher Place United States
Journal Environmental and Molecular Mutagenesis
Volume Number 25
Issue Number Suppl25
Technical Publication No. CONF-9503160-


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