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Author Lai, Fang-Nong ♦ Ma, Jun-Yu ♦ Liu, Jing-Cai ♦ Wang, Jun-Jie ♦ Cheng, Shun-Feng ♦ Sun, Xiao-Feng ♦ Li, Lan ♦ Li, Bo ♦ Nyachoti, Charles Martin ♦ Shen, Wei
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword APPLIED LIFE SCIENCES ♦ CYSTEINE ♦ ESTROGENS ♦ EXTRUSION ♦ FUSARIUM ♦ GLUTATHIONE ♦ IN VITRO ♦ INHIBITION ♦ MATURATION ♦ MESSENGER-RNA ♦ MORPHOLOGY ♦ MYCOTOXINS ♦ OOCYTES ♦ REPRODUCTION
Abstract Zearalenone (ZEA), one of the mycotoxins produced by Fusarium fungi, impacts porcine reproduction by interfering with the estrogen signaling pathway. Previous studies have shown that ZEA inhibits porcine oocyte maturation through the formation of aberrant spindle. To explore the effect of ZEA on porcine oocyte meiotic maturation, the extent of both nuclear and cytoplasmic maturation was examined in this study. Compared with control group, presence of ZEA (3 μM) during oocyte maturation, significantly inhibited the polar body extrusions from 71% to 51%, and significantly increased intracellular reactive oxygen species (ROS) level (12.01 vs. 5.89). Intracellular glutathione (GSH) content in ZEA treatment group was lower than in the control group (1.08 pmol/oocyte vs. 0.18 pmol/oocyte), and cortical granules of cortical area distributed oocytes were reduced (88% vs. 62%). ZEA decreases cumulus expansion in both morphology and mRNA level (HAS2, PTX3, TNFAIP6 and CX43). Addition of N-acetyl-L-cysteine (NAC) to the oocyte maturation media reversed the ZEA-induced inhibition of polar body extrusion (from 69% to 81%), up-regulated ROS (from 7.9 to 6.5), down-regulated GSH content (from 0.16 to 0.82 pmol/oocyte) and recovered cumulus cells expansion in morphology and mRNA level. It is concluded that ZEA affects both oocyte nucleus and cytoplasmic maturation during in vitro maturation, and NAC can reverse these damages to some extent. - Highlights: • ZEA significantly inhibited the polar body extrusions during oocyte maturation. • ZEA significantly increased intracellular ROS level and reduced GSH content. • ZEA disturbed cortical granules of cortical area distributed oocytes. • NAC reversed the ZEA-induced inhibition of oocyte maturation.
ISSN 0041008X
Educational Use Research
Learning Resource Type Article
Publisher Date 2015-12-01
Publisher Place United States
Journal Toxicology and Applied Pharmacology
Volume Number 289
Issue Number 2


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