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Author Mochizuki, D. Y. ♦ Eisenman, J. R. ♦ Conlon, P. J. ♦ Park, L. S. ♦ Urdal, D. L.
Source United States Department of Energy Office of Scientific and Technical Information
Content type Text
Language English
Subject Keyword BASIC BIOLOGICAL SCIENCES ♦ BLOOD FORMATION ♦ BIOLOGICAL PATHWAYS ♦ GLUCOPROTEINS ♦ RADIOIMMUNOASSAY ♦ IMMUNE SERUMS ♦ CHEMICAL PREPARATION ♦ BONE MARROW CELLS ♦ CELL PROLIFERATION ♦ CLONE CELLS ♦ COLONY FORMATION ♦ DNA ♦ INHIBITION ♦ IODINE 125 ♦ LEUKOCYTES ♦ LYMPHOKINES ♦ MACROPHAGES ♦ MICE ♦ STIMULATION ♦ YEASTS ♦ ANIMAL CELLS ♦ ANIMALS ♦ BETA DECAY RADIOISOTOPES ♦ BIOLOGICAL MATERIALS ♦ BLOOD ♦ BLOOD CELLS ♦ BODY FLUIDS ♦ CARBOHYDRATES ♦ CELL CULTURES ♦ CONNECTIVE TISSUE CELLS ♦ DAYS LIVING RADIOISOTOPES ♦ ELECTRON CAPTURE RADIOISOTOPES ♦ FUNGI ♦ GROWTH FACTORS ♦ IMMUNOASSAY ♦ IMMUNOLOGY ♦ INTERMEDIATE MASS NUCLEI ♦ IODINE ISOTOPES ♦ ISOTOPE APPLICATIONS ♦ ISOTOPES ♦ MAMMALS ♦ MATERIALS ♦ MICROORGANISMS ♦ MITOGENS ♦ NUCLEI ♦ NUCLEIC ACIDS ♦ ODD-EVEN NUCLEI ♦ ORGANIC COMPOUNDS ♦ PHAGOCYTES ♦ PLANTS ♦ PROTEINS ♦ RADIOASSAY ♦ RADIOIMMUNOLOGY ♦ RADIOISOTOPES ♦ RODENTS ♦ SACCHARIDES ♦ SOMATIC CELLS ♦ SYNTHESIS ♦ TRACER TECHNIQUES ♦ VERTEBRATES ♦ Physiological Systems- Tracer Techniques
Abstract The expression in yeast of a cDNA clone encoding murine granulocyte-macrophage colony-stimulating factor (GM-CSF) has made possible the purification of large quantities of this recombinant protein. Rabbits immunized with pure recombinant GM-CSF generated antibodies that were shown to be specific for both recombinant GM-CSF and GM-CSF isolated from natural sources. Other lymphokines, including IL 1..beta.., IL 2, IL 3, and recombinant human GM-CSF did not react with the antiserum. The antiserum together with recombinant GM-CSF that had been radiolabeled with /sup 125/I to high specific activity, formed the foundation for a rapid, sensitive, and quantitative radioimmunoassay specific for murine GM-CSF. Furthermore, the antiserum was found to inhibit the biologic activities of GM-CSF as measured in both a bone marrow proliferation assay and a colony assay, and thus should prove to be a useful reagent for dissecting the complex growth factor activities involved in murine hematopoiesis.
Educational Use Research
Learning Resource Type Article
Publisher Date 1986-05-15
Publisher Place United States
Journal J. Immunol.
Volume Number 136
Issue Number 10
Organization Immunex Corp., Seattle, WA


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